000132156 001__ 132156 000132156 005__ 20250923084416.0 000132156 0247_ $$2doi$$a10.1039/d3fo05250h 000132156 0248_ $$2sideral$$a137390 000132156 037__ $$aART-2024-137390 000132156 041__ $$aeng 000132156 100__ $$aGraikini, Dimitra$$uUniversidad de Zaragoza 000132156 245__ $$aAntirotaviral activity of dairy byproducts enriched in fractions from hyperimmune bovine colostrum: the effect of thermal and high hydrostatic pressure treatments 000132156 260__ $$c2024 000132156 5060_ $$aAccess copy available to the general public$$fUnrestricted 000132156 5203_ $$aNowadays, rotaviruses remain a major health burden, especially in developing countries, and strategies complementary to vaccination are needed. In this view, dairy fractions have attracted great scientific interest, due to their high content of bioactive compounds. The objective of this study was to evaluate the antiviral activity of whey and buttermilk enriched in proteins from hyperimmune bovine colostrum (HBC) against rotavirus. The enriched fractions were spray-dried and subsequently tested for their neutralizing activity against the bovine rotavirus WC3 strain in vitro, using differentiated Caco-2/TC7 cells. The highest antirotaviral activity was observed when whey and buttermilk were enriched in purified immunoglobulin G (IgG), showing complete rotavirus neutralization at concentrations of 3 and 6 mg mL−1 for whey and buttermilk, respectively. Additionally, the use of a crude immunoglobulin fraction also gave satisfactory results. The inhibitory activities of all samples significantly decreased after the application of heat, except for the IgG-enriched buttermilk which showed a slight increase of activity following the application of short-time treatments (75 or 85 °C for 20 s). This sample also showed a significant increase of activity (13%) after the application of low-intensity high hydrostatic pressure treatment (400 MPa for 5 min). The maximum loss of bioactivity was observed at 600 MPa for 10 min (31 and 20% for whey- and buttermilk-based formulas, respectively). This study provides relevant information on the potential of whey, buttermilk, and HBC to be part of functional products as complementary strategies to combat rotavirus infections. 000132156 536__ $$9info:eu-repo/grantAgreement/EC/H2020/801586/EU/International Doctoral Programme for Talent Attraction to the Campus of International Excellence of the Ebro Valley/IberusTalent$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 801586-IberusTalent$$9info:eu-repo/grantAgreement/ES/MICINN/PID2022-139104OB-I00$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2017-82987-R 000132156 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/ 000132156 590__ $$a5.4$$b2024 000132156 592__ $$a1.194$$b2024 000132156 591__ $$aFOOD SCIENCE & TECHNOLOGY$$b38 / 181 = 0.21$$c2024$$dQ1$$eT1 000132156 593__ $$aMedicine (miscellaneous)$$c2024$$dQ1 000132156 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b61 / 319 = 0.191$$c2024$$dQ1$$eT1 000132156 593__ $$aFood Science$$c2024$$dQ1 000132156 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000132156 700__ $$aGarcía, Laura$$uUniversidad de Zaragoza 000132156 700__ $$aAbad, Inés$$uUniversidad de Zaragoza 000132156 700__ $$aLavilla, María 000132156 700__ $$aPuértolas, Eduardo 000132156 700__ $$0(orcid)0000-0003-2555-8425$$aPérez, María Dolores$$uUniversidad de Zaragoza 000132156 700__ $$0(orcid)0000-0001-5964-823X$$aSánchez, Lourdes$$uUniversidad de Zaragoza 000132156 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos 000132156 773__ $$g15, 4 (2024), 2265-2281$$pFOOD & FUNCTION$$tFOOD & FUNCTION$$x2042-6496 000132156 8564_ $$s3444076$$uhttps://zaguan.unizar.es/record/132156/files/texto_completo.pdf$$yVersión publicada 000132156 8564_ $$s2668593$$uhttps://zaguan.unizar.es/record/132156/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000132156 909CO $$ooai:zaguan.unizar.es:132156$$particulos$$pdriver 000132156 951__ $$a2025-09-22-14:33:07 000132156 980__ $$aARTICLE