000132174 001__ 132174
000132174 005__ 20241125101141.0
000132174 0247_ $$2doi$$a10.3390/ijms25010009
000132174 0248_ $$2sideral$$a137453
000132174 037__ $$aART-2023-137453
000132174 041__ $$aeng
000132174 100__ $$aMunuera, Ines
000132174 245__ $$aChronic Glaucoma Induced in Rats by a Single Injection of Fibronectin-Loaded PLGA Microspheres: IOP-Dependent and IOP-Independent Neurodegeneration
000132174 260__ $$c2023
000132174 5060_ $$aAccess copy available to the general public$$fUnrestricted
000132174 5203_ $$aTo evaluate a new animal model of chronic glaucoma induced using a single injection of fibronectin-loaded biodegradable PLGA microspheres (Ms) to test prolonged therapies. 30 rats received a single injection of fibronectin-PLGA-Ms suspension (MsF) in the right eye, 10 received non-loaded PLGA-Ms suspension (Control), and 17 were non-injected (Healthy). Follow-up was performed (24 weeks), evaluating intraocular pressure (IOP), optical coherence tomography (OCT), histology and electroretinography. The right eyes underwent a progressive increase in IOP, but only induced cohorts reached hypertensive values. The three cohorts presented a progressive decrease in ganglion cell layer (GCL) thickness, corroborating physiological age-related loss of ganglion cells. Injected cohorts (MsF > Control) presented greater final GCL thickness. Histological exams explain this paradox: the MsF cohort showed lower ganglion cell counts but higher astrogliosis and immune response. A sequential trend of functional damage was recorded using scotopic electroretinography (MsF > Control > Healthy). It seems to be a function–structure correlation: in significant astrogliosis, early functional damage can be detected by electroretinography, and structural damage can be detected by histological exams but not by OCT. Males presented higher IOP and retinal and GCL thicknesses and lower electroretinography. A minimally invasive chronic glaucoma model was induced by a single injection of biodegradable Ms.
000132174 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/JR22-00057$$9info:eu-repo/grantAgreement/ES/ISCIII/M17-00213$$9info:eu-repo/grantAgreement/ES/ISCIII/PI17-01726$$9info:eu-repo/grantAgreement/ES/ISCIII/PI17-01946$$9info:eu-repo/grantAgreement/ES/ISCIII/PI20-00437$$9info:eu-repo/grantAgreement/ES/MCIN/AEI/10.13039/501100011033$$9info:eu-repo/grantAgreement/ES/MICINN/PRE2018-083951$$9info:eu-repo/grantAgreement/ES/MICINN/RYC2020-029929-I$$9info:eu-repo/grantAgreement/ES/MINECO-AEI-FEDER/PID2020-113281RB-C21$$9info:eu-repo/grantAgreement/ES/MINECO-AEI-FEDER/PID2020-113281RB-C22
000132174 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000132174 590__ $$a4.9$$b2023
000132174 592__ $$a1.179$$b2023
000132174 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b66 / 313 = 0.211$$c2023$$dQ1$$eT1
000132174 593__ $$aMedicine (miscellaneous)$$c2023$$dQ1
000132174 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b68 / 231 = 0.294$$c2023$$dQ2$$eT1
000132174 593__ $$aPhysical and Theoretical Chemistry$$c2023$$dQ1
000132174 593__ $$aComputer Science Applications$$c2023$$dQ1
000132174 593__ $$aInorganic Chemistry$$c2023$$dQ1
000132174 593__ $$aSpectroscopy$$c2023$$dQ1
000132174 593__ $$aOrganic Chemistry$$c2023$$dQ1
000132174 593__ $$aMolecular Biology$$c2023$$dQ2
000132174 593__ $$aCatalysis$$c2023$$dQ2
000132174 594__ $$a8.1$$b2023
000132174 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000132174 700__ $$aAragon-Navas, Alba
000132174 700__ $$aVillacampa, Pilar
000132174 700__ $$aGonzalez-Cela, Miriam A.
000132174 700__ $$aSubías, Manuel$$uUniversidad de Zaragoza
000132174 700__ $$0(orcid)0000-0003-2389-8282$$aPablo, Luis E.$$uUniversidad de Zaragoza
000132174 700__ $$aGarcia-Feijoo, Julian
000132174 700__ $$aHerrero-Vanrell, Rocio
000132174 700__ $$0(orcid)0000-0001-6258-2489$$aGarcia-Martin, Elena$$uUniversidad de Zaragoza
000132174 700__ $$aBravo-Osuna, Irene
000132174 700__ $$aRodrigo, Maria J.$$uUniversidad de Zaragoza
000132174 7102_ $$11013$$2646$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Oftalmología
000132174 773__ $$g25, 1 (2023), 9 [27 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000132174 8564_ $$s16457392$$uhttps://zaguan.unizar.es/record/132174/files/texto_completo.pdf$$yVersión publicada
000132174 8564_ $$s2610064$$uhttps://zaguan.unizar.es/record/132174/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000132174 909CO $$ooai:zaguan.unizar.es:132174$$particulos$$pdriver
000132174 951__ $$a2024-11-22-12:02:51
000132174 980__ $$aARTICLE