000132184 001__ 132184
000132184 005__ 20260112133159.0
000132184 0247_ $$2doi$$a10.3390/diagnostics14030319
000132184 0248_ $$2sideral$$a137498
000132184 037__ $$aART-2024-137498
000132184 041__ $$aeng
000132184 100__ $$aDashti, Alejandro
000132184 245__ $$aEvaluation of the use of Singleplex and Duplex CerTest VIASURE Real-Time PCR Assays to detect common intestinal protist parasites
000132184 260__ $$c2024
000132184 5060_ $$aAccess copy available to the general public$$fUnrestricted
000132184 5203_ $$aCryptosporidium spp., Giardia duodenalis and Entamoeba histolytica are species of protozoa- causing diarrhoea that are common worldwide, while Entamoeba dispar, Dientamoeba fragilis and Blastocystis sp. appear to be commensal parasites whose role in pathogenicity remains controversial. We conducted the clinical evaluation of five singleplex and one duplex CerTest VIASURE Real-Time PCR Assays against a large panel of positive DNA samples (n = 358), and specifically to Cryptosporidium spp. (n = 96), G. duodenalis (n = 115), E. histolytica (n = 25) E. dispar (n = 11), Blastocystis sp. (n = 42), D. fragilis (n = 37), and related parasitic phylum species such as Apicomplexa, Euglenozoa, Microsporidia and Nematoda. DNA samples were obtained from clinical stool specimens or cultured isolates in a national reference centre. Estimated diagnostic sensitivity and specificity values were 0.94–1 for Cryptosporidium spp., 0.96–0.99 for G. duodenalis, 0.96–1 for E. histolytica, 1–1 for E. dispar, and 1–0.99 for D. fragilis in the evaluated singleplex assays. In the duplex assay for the simultaneous detection of Blastocystis sp. and D. fragilis these values were 1–0.98 and 1–0.99, respectively. Measures of diagnostic precision for repeatability and reproducibility were found to be under acceptable ranges. The assays identified six Cryptosporidium species (C. hominis, C. parvum, C. canis, C. felis, C. scrofarum, and C. ryanae), four G. duodenalis assemblages (A, B, C, and F), and six Blastocystis subtypes (ST1-ST5, and ST8). The evaluated singleplex and duplex VIASURE Real-Time PCR assays provide sensitive, practical, and cost-effective choices to the molecular diagnosis of the main diarrhoea-causing intestinal protists in clinical microbiology and research laboratories.
000132184 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/PI17-2017-v3
000132184 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000132184 590__ $$a3.3$$b2024
000132184 592__ $$a0.773$$b2024
000132184 591__ $$aMEDICINE, GENERAL & INTERNAL$$b57 / 332 = 0.172$$c2024$$dQ1$$eT1
000132184 593__ $$aInternal Medicine$$c2024$$dQ2
000132184 593__ $$aClinical Biochemistry$$c2024$$dQ2
000132184 594__ $$a5.9$$b2024
000132184 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000132184 700__ $$0(orcid)0000-0003-2742-8827$$aAlonso, Henar
000132184 700__ $$aEscolar-Miñana, Cristina
000132184 700__ $$aKöster, Pamela C.
000132184 700__ $$aBailo, Begoña
000132184 700__ $$aCarmena, David
000132184 700__ $$aGonzález-Barrio, David
000132184 773__ $$g14, 319 (2024), 1-13$$tDiagnostics$$x2075-4418
000132184 8564_ $$s294495$$uhttps://zaguan.unizar.es/record/132184/files/texto_completo.pdf$$yVersión publicada
000132184 8564_ $$s2727345$$uhttps://zaguan.unizar.es/record/132184/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000132184 909CO $$ooai:zaguan.unizar.es:132184$$particulos$$pdriver
000132184 951__ $$a2026-01-12-12:39:01
000132184 980__ $$aARTICLE