000132187 001__ 132187
000132187 005__ 20240301161206.0
000132187 0247_ $$2doi$$a10.3390/ani14020252
000132187 0248_ $$2sideral$$a137513
000132187 037__ $$aART-2024-137513
000132187 041__ $$aeng
000132187 100__ $$aGarcía-Gracia, Mireya
000132187 245__ $$aAnalysis of plasma-derived exosomal microRNAs as potential biomarkers for canine idiopathic epilepsy
000132187 260__ $$c2024
000132187 5060_ $$aAccess copy available to the general public$$fUnrestricted
000132187 5203_ $$aEpilepsy is one of the most prevalent complex neurological diseases in both the canine and human species, with the idiopathic form as its most common diagnosis. MicroRNAs (miRNAs) are small, noncoding RNA molecules that play a role in gene regulation processes and appear to be a promising biological target for convulsion control. These molecules have been reported as constituents of the internal content of exosomes, which are small extracellular vesicles released by cells. In this study, exosome samples were isolated from the plasma of 23 dogs, including 9 dogs with epilepsy responsive to treatment, 6 dogs with drug-resistant epilepsy, and 8 control dogs. Plasma exosomes were then characterized by electron transmission microscopy, nanoparticle tracking analysis, and dot blotting. Afterwards, the microRNA-enriched RNA content of exosomes was isolated, and miRNA quantification was performed by quantitative real-time PCR. Seven circulating miRNAs that have been previously described in the literature as potential diagnostic or prognostic biomarkers for epilepsy were evaluated. We observed significant differences in miR-16 (p < 0.001), miR-93-5p (p < 0.001), miR-142 (p < 0.001), miR-574 (p < 0.01), and miR-27 (p < 0.05) levels in dogs with refractory epilepsy compared to the control group. In drug-sensitive epileptic dogs, miR-142 (p < 0.01) showed significant differences compared to healthy dogs. Moreover, distinct levels of miR-16 (p < 0.05), miR-93-5p (p < 0.01), miR-132 (p < 0.05), and miR-574 (p < 0.05) were also found between drug-sensitive and drug-resistant epileptic dogs. Our results present plasma-circulating exosomes as an advantageous source of epileptic biomarkers, highlighting the potential of miRNAs as prognostic and diagnostic biomarkers of canine idiopathic epilepsy.
000132187 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A19-23R$$9info:eu-repo/grantAgreement/ES/DGA/LMP134-21$$9info:eu-repo/grantAgreement/ES/ISCIII/CB18-05-0037$$9info:eu-repo/grantAgreement/ES/ISCIII/FEDER/PI21-00372
000132187 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000132187 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000132187 700__ $$0(orcid)0000-0002-7277-4318$$aMoreno-Martinez, Laura$$uUniversidad de Zaragoza
000132187 700__ $$0(orcid)0000-0002-6806-9990$$aHernaiz, Adelaida$$uUniversidad de Zaragoza
000132187 700__ $$aUsón, Sebastián
000132187 700__ $$aMoral, Jon
000132187 700__ $$0(orcid)0000-0002-5228-248X$$aSanz-Rubio, David
000132187 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, Pilar$$uUniversidad de Zaragoza
000132187 700__ $$0(orcid)0000-0002-1581-3085$$aPalacio, Jorge$$uUniversidad de Zaragoza
000132187 700__ $$0(orcid)0000-0003-1728-900X$$aRosado, Belén$$uUniversidad de Zaragoza
000132187 700__ $$0(orcid)0000-0001-5687-6704$$aOsta, Rosario$$uUniversidad de Zaragoza
000132187 700__ $$aGarcía-Belenguer, Sylvia
000132187 700__ $$0(orcid)0000-0001-6016-4726$$aMartín Burriel, Inmaculada$$uUniversidad de Zaragoza
000132187 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000132187 7102_ $$11012$$2315$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Farmacología
000132187 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000132187 773__ $$g14, 2 (2024), 252 [15 pp.]$$pAnimals (Basel)$$tAnimals$$x2076-2615
000132187 8564_ $$s2209199$$uhttps://zaguan.unizar.es/record/132187/files/texto_completo.pdf$$yVersión publicada
000132187 8564_ $$s2865452$$uhttps://zaguan.unizar.es/record/132187/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000132187 909CO $$ooai:zaguan.unizar.es:132187$$particulos$$pdriver
000132187 951__ $$a2024-03-01-14:51:38
000132187 980__ $$aARTICLE