000132212 001__ 132212
000132212 005__ 20240705134156.0
000132212 0247_ $$2doi$$a10.1152/ajpgi.00264.2023
000132212 0248_ $$2sideral$$a137346
000132212 037__ $$aART-2024-137346
000132212 041__ $$aeng
000132212 100__ $$0(orcid)0000-0002-4665-9674$$aHerrera-Marcos, Luis V.$$uUniversidad de Zaragoza
000132212 245__ $$aLipidomic signatures discriminate subtle hepatic changes in the progression of porcine non-alcoholic steatohepatitis
000132212 260__ $$c2024
000132212 5060_ $$aAccess copy available to the general public$$fUnrestricted
000132212 5203_ $$aRecently, the development of non-alcoholic steatohepatitis (NASH) in common strains of pigs has been achieved using a diet high in saturated fat, fructose, cholesterol and cholate and deficient in choline and methionine. The aim of the present work was to characterize the hepatic and plasma lipidomic changes that accompany the progression of NASH and its reversal by switching pigs back to a chow diet. One month of this extreme steatotic diet was sufficient to induce porcine NASH. The lipidomic platform using liquid chromatography-mass spectrometry analyzed 467 lipid species. Seven hepatic phospholipids [PC(30:0), PC(32:0), PC(33:0), PC(33:1), PC(34:0), PC(34:3) and PC(36:2)] significantly discriminated the time of dietary exposure, and PC(30:0), PC(33:0), PC(33:1) and PC(34:0) showed rapid adaptation in the reversion period. Three transcripts, CS, MAT1A and SPP1, showed significant changes associated with hepatic triglycerides and PC(33:0). Plasma lipidomics revealed that these species [FA 16:0, FA 18:0, LPC(17:1), PA(40:5), PC(37:1), TG(45:0), TG(47:2) and TG(51:0)] were able to discriminate the time of dietary exposure. Among them, FA 16:0, FA 18:0, LPC(17:1) and PA(40:5) changed the trend in the reversion phase. Plasma LDL-cholesterol and IL12P40 were good parameters to study the progression of NASH, but their capacity was surpassed by hepatic [PC(33:0), PC(33:1), and PC(34:0)] or plasma lipid [FA 16:0, FA 18:0, and LPC(17:1)] species. Taken together, these lipid species can be used as biomarkers of metabolic changes in the progression and regression of NASH in this model. The lipid changes suggest that the development of NASH also affects peripheral lipid metabolism.
000132212 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B16-23R$$9info:eu-repo/grantAgreement/ES/ISCIII-CIBERObn/CB06-03-1012$$9info:eu-repo/grantAgreement/ES/MICINN AEI/PID2019-104915RB-I00$$9info:eu-repo/grantAgreement/ES/MICINN AEI/PID2022-104915RB-I00$$9info:eu-repo/grantAgreement/ES/MINECO/SAF2016-75441-R
000132212 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000132212 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000132212 700__ $$0(orcid)0000-0002-8100-5596$$aMartínez-Beamonte, Roberto$$uUniversidad de Zaragoza
000132212 700__ $$0(orcid)0000-0003-1197-4276$$aArnal, Carmen
000132212 700__ $$0(orcid)0000-0002-8442-8041$$aBarranquero, Cristina
000132212 700__ $$0(orcid)0000-0002-7213-7095$$aPuente-Lanzarote, Juan J.
000132212 700__ $$0(orcid)0000-0001-9578-6525$$aLou-Bonafonte, José M.$$uUniversidad de Zaragoza
000132212 700__ $$0(orcid)0009-0008-6478-7857$$aGonzalo-Romeo, Gonzalo$$uUniversidad de Zaragoza
000132212 700__ $$aMocciaro, Gabriele
000132212 700__ $$aJenkins, Benjamin
000132212 700__ $$0(orcid)0000-0002-5841-0462$$aSurra, Joaquin C.$$uUniversidad de Zaragoza
000132212 700__ $$0(orcid)0000-0002-3595-7668$$aRodríguez-Yoldi, María J.$$uUniversidad de Zaragoza
000132212 700__ $$aAlastrué-Vera, Víctor
000132212 700__ $$aLetosa, Jesús
000132212 700__ $$0(orcid)0000-0002-8275-7191$$aGarcía-Gil, Agustín
000132212 700__ $$0(orcid)0000-0002-4437-2581$$aGüemes, Antonio$$uUniversidad de Zaragoza
000132212 700__ $$aKoulman, Albert
000132212 700__ $$0(orcid)0000-0002-8251-8457$$aOsada, Jesús$$uUniversidad de Zaragoza
000132212 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000132212 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000132212 7102_ $$11013$$2090$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Cirugía
000132212 7102_ $$10$$2X$$aUniversidad de Zaragoza$$bServ.Gral. Apoyo Investigación$$cServ. Div. Experimenta. Animal
000132212 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000132212 7102_ $$11002$$2050$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Biología Celular
000132212 773__ $$g326, 4 (2024), G411-G425$$pAm. j. physiol.: gasterointest. liver physiol.$$tAMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY$$x0193-1857
000132212 8564_ $$s1227564$$uhttps://zaguan.unizar.es/record/132212/files/texto_completo.pdf$$yPostprint$$zinfo:eu-repo/date/embargoEnd/2025-02-20
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000132212 909CO $$ooai:zaguan.unizar.es:132212$$particulos$$pdriver
000132212 951__ $$a2024-07-05-12:49:50
000132212 980__ $$aARTICLE