132212 20260218073116.0 doi 10.1152/ajpgi.00264.2023 sideral 137346 ART-2024-137346 eng (orcid)0000-0002-4665-9674 Herrera-Marcos, Luis V. Universidad de Zaragoza Lipidomic signatures discriminate subtle hepatic changes in the progression of porcine non-alcoholic steatohepatitis 2024 Access copy available to the general public Unrestricted Recently, the development of non-alcoholic steatohepatitis (NASH) in common strains of pigs has been achieved using a diet high in saturated fat, fructose, cholesterol and cholate and deficient in choline and methionine. The aim of the present work was to characterize the hepatic and plasma lipidomic changes that accompany the progression of NASH and its reversal by switching pigs back to a chow diet. One month of this extreme steatotic diet was sufficient to induce porcine NASH. The lipidomic platform using liquid chromatography-mass spectrometry analyzed 467 lipid species. Seven hepatic phospholipids [PC(30:0), PC(32:0), PC(33:0), PC(33:1), PC(34:0), PC(34:3) and PC(36:2)] significantly discriminated the time of dietary exposure, and PC(30:0), PC(33:0), PC(33:1) and PC(34:0) showed rapid adaptation in the reversion period. Three transcripts, CS, MAT1A and SPP1, showed significant changes associated with hepatic triglycerides and PC(33:0). Plasma lipidomics revealed that these species [FA 16:0, FA 18:0, LPC(17:1), PA(40:5), PC(37:1), TG(45:0), TG(47:2) and TG(51:0)] were able to discriminate the time of dietary exposure. Among them, FA 16:0, FA 18:0, LPC(17:1) and PA(40:5) changed the trend in the reversion phase. Plasma LDL-cholesterol and IL12P40 were good parameters to study the progression of NASH, but their capacity was surpassed by hepatic [PC(33:0), PC(33:1), and PC(34:0)] or plasma lipid [FA 16:0, FA 18:0, and LPC(17:1)] species. Taken together, these lipid species can be used as biomarkers of metabolic changes in the progression and regression of NASH in this model. The lipid changes suggest that the development of NASH also affects peripheral lipid metabolism. info:eu-repo/grantAgreement/ES/DGA/B16-23R info:eu-repo/grantAgreement/ES/ISCIII-CIBERObn/CB06-03-1012 info:eu-repo/grantAgreement/ES/MICINN AEI/PID2019-104915RB-I00 info:eu-repo/grantAgreement/ES/MICINN AEI/PID2022-104915RB-I00 info:eu-repo/grantAgreement/ES/MINECO/SAF2016-75441-R info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 3.3 2024 PHYSIOLOGY 22 / 87 = 0.253 2024 Q2 T1 GASTROENTEROLOGY & HEPATOLOGY 50 / 148 = 0.338 2024 Q2 T2 1.39 2024 Gastroenterology 2024 Q1 Physiology (medical) 2024 Q1 Physiology 2024 Q1 Hepatology 2024 Q1 7.6 2024 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion (orcid)0000-0002-8100-5596 Martínez-Beamonte, Roberto Universidad de Zaragoza (orcid)0000-0003-1197-4276 Arnal, Carmen (orcid)0000-0002-8442-8041 Barranquero, Cristina (orcid)0000-0002-7213-7095 Puente-Lanzarote, Juan J. (orcid)0000-0001-9578-6525 Lou-Bonafonte, José M. Universidad de Zaragoza (orcid)0009-0008-6478-7857 Gonzalo-Romeo, Gonzalo Universidad de Zaragoza Mocciaro, Gabriele Jenkins, Benjamin (orcid)0000-0002-5841-0462 Surra, Joaquin C. Universidad de Zaragoza (orcid)0000-0002-3595-7668 Rodríguez-Yoldi, María J. Universidad de Zaragoza Alastrué-Vera, Víctor Letosa, Jesús (orcid)0000-0002-8275-7191 García-Gil, Agustín (orcid)0000-0002-4437-2581 Güemes, Antonio Universidad de Zaragoza Koulman, Albert (orcid)0000-0002-8251-8457 Osada, Jesús Universidad de Zaragoza 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 1003 027 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Anatom.Embriol.Humana 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 1013 090 Universidad de Zaragoza Dpto. Cirugía Área Cirugía 0 X Universidad de Zaragoza Serv.Gral. Apoyo Investigación Serv. Div. Experimenta. Animal 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 1002 050 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Biología Celular 326, 4 (2024), G411-G425 Am. j. physiol.: gasterointest. liver physiol. AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY 0193-1857 1227564 http://zaguan.unizar.es/record/132212/files/texto_completo.pdf Postprint info:eu-repo/date/embargoEnd/2025-02-20 1758265 http://zaguan.unizar.es/record/132212/files/texto_completo.jpg?subformat=icon icon Postprint info:eu-repo/date/embargoEnd/2025-02-20 oai:zaguan.unizar.es:132212 articulos driver 2026-02-17-20:20:42 ARTICLE