000132249 001__ 132249
000132249 005__ 20260217205505.0
000132249 0247_ $$2doi$$a10.1128/mbio.03231-23
000132249 0248_ $$2sideral$$a137086
000132249 037__ $$aART-2024-137086
000132249 041__ $$aeng
000132249 100__ $$0(orcid)0000-0002-1960-2672$$aSarasa-Buisan, Cristina
000132249 245__ $$aFurC (PerR) contributes to the regulation of peptidoglycan remodeling and intercellular molecular transfer in the cyanobacterium Anabaena sp. strain PCC 7120
000132249 260__ $$c2024
000132249 5060_ $$aAccess copy available to the general public$$fUnrestricted
000132249 5203_ $$aMicrobial extracellular proteins and metabolites provide valuable information concerning how microbes adapt to changing environments. In cyanobacteria, dynamic acclimation strategies involve a variety of regulatory mechanisms, being ferric uptake regulator proteins as key players in this process. In the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120, FurC (PerR) is a global regulator that modulates the peroxide response and several genes involved in photosynthesis and nitrogen metabolism. To investigate the possible role of FurC in shaping the extracellular environment of Anabaena, the analysis of the extracellular metabolites and proteins of a furC-overexpressing variant was compared to that of the wild-type strain. There were 96 differentially abundant proteins, 78 of which were found for the first time in the extracellular fraction of Anabaena. While these proteins belong to different functional categories, most of them are predicted to be secreted or have a peripheral location. Several stress-related proteins, including PrxA, flavodoxin, and the Dps homolog All1173, accumulated in the exoproteome of furC-overexpressing cells, while decreased levels of FurA and a subset of membrane proteins, including several export proteins and amiC gene products, responsible for nanopore formation, were detected. Direct repression by FurC of some of those genes, including amiC1 and amiC2, could account for odd septal nanopore formation and impaired intercellular molecular transfer observed in the furC-overexpressing variant. Assessment of the exometabolome from both strains revealed the release of two peptidoglycan fragments in furC-overexpressing cells, namely 1,6-anhydro-N-acetyl-β-D-muramic acid (anhydroMurNAc) and its associated disaccharide (β-D-GlcNAc-(1-4)-anhydroMurNAc), suggesting alterations in peptidoglycan breakdown and recycling.
000132249 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E35-20R$$9info:eu-repo/grantAgreement/ES/MCIU/PID2019-104889GB-I00$$9info:eu-repo/grantAgreement/ES/MICIU/PID2020-118595GB-I00
000132249 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000132249 590__ $$a4.7$$b2024
000132249 592__ $$a1.885$$b2024
000132249 591__ $$aMICROBIOLOGY$$b33 / 163 = 0.202$$c2024$$dQ1$$eT1
000132249 593__ $$aVirology$$c2024$$dQ1
000132249 593__ $$aMicrobiology$$c2024$$dQ1
000132249 594__ $$a9.0$$b2024
000132249 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000132249 700__ $$aNieves-Morión, Mercedes
000132249 700__ $$aArévalo, Sergio
000132249 700__ $$aHelm, Richard F.
000132249 700__ $$aSevilla, Emma
000132249 700__ $$aLuque, Ignacio
000132249 700__ $$aFillat, María F.
000132249 773__ $$g15, 3 (2024), e03231-23$$pMBIO$$tMBIO$$x2161-2129
000132249 8564_ $$s1168290$$uhttps://zaguan.unizar.es/record/132249/files/texto_completo.pdf$$yVersión publicada
000132249 8564_ $$s2307151$$uhttps://zaguan.unizar.es/record/132249/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000132249 909CO $$ooai:zaguan.unizar.es:132249$$particulos$$pdriver
000132249 951__ $$a2026-02-17-20:23:19
000132249 980__ $$aARTICLE