000132284 001__ 132284
000132284 005__ 20240731103405.0
000132284 0247_ $$2doi$$a10.1039/d3bm01987j
000132284 0248_ $$2sideral$$a137531
000132284 037__ $$aART-2024-137531
000132284 041__ $$aeng
000132284 100__ $$aPostigo, Alejandro
000132284 245__ $$aAssessing the influence of small structural modifications in simple DNA-based nanostructures on their role as drug nanocarriers
000132284 260__ $$c2024
000132284 5060_ $$aAccess copy available to the general public$$fUnrestricted
000132284 5203_ $$aDNA nanotechnology leverages Watson–Crick–Franklin base-pairing interactions to build complex DNA-based nanostructures (DNS). Due to DNA specific self-assembly properties, DNS can be designed with a total control of their architecture, which has been demonstrated to have an impact on the overall DNS features. Indeed, structural properties such as the shape, size and flexibility of DNS can influence their biostability as well as their ability to internalise into cells. We present here two series of simple DNS with small and precise variations related to their length or flexibility and study the influence that these structural changes have on their overall properties as drug nanocarriers. Results indicate that shorter and more flexible DNS present higher stability towards nuclease degradation. These structural changes also have a certain effect on their cell internalisation ability and drug release rate. Consequently, drug-loaded DNS cytotoxicity varies according to the design, with lower cell viability values obtained in the DNS exhibiting faster drug release and larger cell interaction rates. In summary, small changes in the structure of simple DNS can have an influence on their overall capabilities as drug nanocarriers. The effects reported here could guide the design of simple DNS for future therapeutic uses.
000132284 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E47-23R$$9info:eu-repo/grantAgreement/ES/DGA-FSE/E47-20R$$9info:eu-repo/grantAgreement/ES/DGA/LMP128_21$$9info:eu-repo/grantAgreement/EC/H2020/853468/EU/Remote control of cellular signalling triggered by magnetic switching/SIROCCO$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 853468-SIROCCO$$9info:eu-repo/grantAgreement/ES/MICINN/CTQ2017-84087-R$$9info:eu-repo/grantAgreement/ES/MCINN/PID2020-113003GB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/RYC-2015-18471
000132284 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000132284 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000132284 700__ $$aMartínez-Vicente, Pablo$$uUniversidad de Zaragoza
000132284 700__ $$aBaumann, Kevin N.
000132284 700__ $$0(orcid)0000-0002-5380-6863$$aBarrio, Jesús del$$uUniversidad de Zaragoza
000132284 700__ $$0(orcid)0000-0003-3109-4284$$aHernández-Ainsa, Silvia
000132284 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000132284 7102_ $$11002$$2807$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Toxicología
000132284 773__ $$g12, 6 (2024), 1549-1557$$pBiomater. sci.$$tBiomaterials Science$$x2047-4830
000132284 8564_ $$s1431757$$uhttps://zaguan.unizar.es/record/132284/files/texto_completo.pdf$$yVersión publicada
000132284 8564_ $$s2841071$$uhttps://zaguan.unizar.es/record/132284/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000132284 909CO $$ooai:zaguan.unizar.es:132284$$particulos$$pdriver
000132284 951__ $$a2024-07-31-10:01:17
000132284 980__ $$aARTICLE