000132456 001__ 132456
000132456 005__ 20240311111225.0
000132456 0247_ $$2doi$$a10.1016/j.idairyj.2023.105818
000132456 0248_ $$2sideral$$a137562
000132456 037__ $$aART-2024-137562
000132456 041__ $$aeng
000132456 100__ $$aGraikini, Dimitra$$uUniversidad de Zaragoza
000132456 245__ $$aEvaluation of in vitro antirotaviral activity of lactoferrin from different species using a human intestinal model
000132456 260__ $$c2024
000132456 5060_ $$aAccess copy available to the general public$$fUnrestricted
000132456 5203_ $$aRotaviruses are the leading agents of severe diarrheal disease in infants and young children and complementary strategies to vaccination are needed. Therefore, we evaluated the activity of lactoferrin (LF) from different species against rotavirus in differentiated Caco-2/TC7 cells. The mechanism of rotavirus inhibition and the influence of iron saturation and sialic acid content were also investigated. Furthermore, the effect of bovine LF on Toll-like receptor expression (TLRs) during rotavirus infection was studied. LF presented high inhibitory activity against rotavirus above 8 mg mL−1, the bovine protein being the most potent. The antirotaviral mechanism of LF was mainly related to the virus neutralisation at an early step of infection. Interestingly, a direct relationship between rotavirus neutralisation activity and content of sialic acid in LFs was observed. Finally, the neutralisation of rotavirus by LF leaded to a decreased expression of TLRs in Caco-2/TC7 cells, with a more evident effect on TLR7.
000132456 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A20-23R$$9info:eu-repo/grantAgreement/EC/H2020/801586/EU/International Doctoral Programme for Talent Attraction to the Campus of International Excellence of the Ebro Valley/IberusTalent$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 801586-IberusTalent$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2017-82987-R
000132456 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000132456 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000132456 700__ $$0(orcid)0000-0003-0862-9893$$aConesa, Celia
000132456 700__ $$aAbad, Inés$$uUniversidad de Zaragoza
000132456 700__ $$0(orcid)0000-0003-2555-8425$$aPérez, María Dolores$$uUniversidad de Zaragoza
000132456 700__ $$0(orcid)0000-0001-5964-823X$$aSánchez, Lourdes$$uUniversidad de Zaragoza
000132456 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000132456 773__ $$g149 (2024), 105818 [10 pp.]$$pInt. dairy j.$$tINTERNATIONAL DAIRY JOURNAL$$x0958-6946
000132456 8564_ $$s972585$$uhttps://zaguan.unizar.es/record/132456/files/texto_completo.pdf$$yVersión publicada
000132456 8564_ $$s2822673$$uhttps://zaguan.unizar.es/record/132456/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000132456 909CO $$ooai:zaguan.unizar.es:132456$$particulos$$pdriver
000132456 951__ $$a2024-03-11-09:51:44
000132456 980__ $$aARTICLE