132456 20260217205507.0 doi 10.1016/j.idairyj.2023.105818 sideral 137562 ART-2024-137562 eng Graikini, Dimitra Universidad de Zaragoza Evaluation of in vitro antirotaviral activity of lactoferrin from different species using a human intestinal model 2024 Access copy available to the general public Unrestricted Rotaviruses are the leading agents of severe diarrheal disease in infants and young children and complementary strategies to vaccination are needed. Therefore, we evaluated the activity of lactoferrin (LF) from different species against rotavirus in differentiated Caco-2/TC7 cells. The mechanism of rotavirus inhibition and the influence of iron saturation and sialic acid content were also investigated. Furthermore, the effect of bovine LF on Toll-like receptor expression (TLRs) during rotavirus infection was studied. LF presented high inhibitory activity against rotavirus above 8 mg mL−1, the bovine protein being the most potent. The antirotaviral mechanism of LF was mainly related to the virus neutralisation at an early step of infection. Interestingly, a direct relationship between rotavirus neutralisation activity and content of sialic acid in LFs was observed. Finally, the neutralisation of rotavirus by LF leaded to a decreased expression of TLRs in Caco-2/TC7 cells, with a more evident effect on TLR7. info:eu-repo/grantAgreement/ES/DGA/A20-23R info:eu-repo/grantAgreement/EC/H2020/801586/EU/International Doctoral Programme for Talent Attraction to the Campus of International Excellence of the Ebro Valley/IberusTalent This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 801586-IberusTalent info:eu-repo/grantAgreement/ES/MINECO/AGL2017-82987-R info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 3.4 2024 FOOD SCIENCE & TECHNOLOGY 73 / 182 = 0.401 2024 Q2 T2 0.849 2024 Food Science 2024 Q1 Applied Microbiology and Biotechnology 2024 Q2 7.4 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Conesa, Celia Universidad de Zaragoza (orcid)0000-0003-0862-9893 Abad, Inés Universidad de Zaragoza Pérez, María Dolores Universidad de Zaragoza (orcid)0000-0003-2555-8425 Sánchez, Lourdes Universidad de Zaragoza (orcid)0000-0001-5964-823X 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 149 (2024), 105818 [10 pp.] Int. dairy j. INTERNATIONAL DAIRY JOURNAL 0958-6946 972585 http://zaguan.unizar.es/record/132456/files/texto_completo.pdf Versión publicada 2822673 http://zaguan.unizar.es/record/132456/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:132456 articulos driver 2026-02-17-20:23:51 ARTICLE