000132473 001__ 132473
000132473 005__ 20240311111225.0
000132473 0247_ $$2doi$$a10.1016/j.cattod.2024.114562
000132473 0248_ $$2sideral$$a137629
000132473 037__ $$aART-2024-137629
000132473 041__ $$aeng
000132473 100__ $$0(orcid)0000-0003-2587-6423$$aMenacho, Carmen
000132473 245__ $$aInactivation of Acanthamoeba and its endosymbiont bacteria by the combination of solar light with H2O2
000132473 260__ $$c2024
000132473 5060_ $$aAccess copy available to the general public$$fUnrestricted
000132473 5203_ $$aDisinfection treatments are necessary for the safe use of water, but Free-Living Amoebae (FLA) are known to resist conventional processes, posing a health threat to water users that increases due to the endosymbiont bacteria (EB) FLA can carry inside. Advanced Oxidation Processes are promising disinfection treatments that have been extensively studied on bacteria but have barely been studied on FLA or EB yet. For the first time, the inactivation efficiency of combining H2O2 and simulated solar radiation (SR) against Acanthamoeba and their EB was evaluated. Its performance was compared to only H2O2 and solar radiation (SR) at 280–800 nm. The influence of Acanthamoeba origin was also evaluated. Concentrations of 7 to 10 mM H2O2 were necessary for inactivating Acanthamoebae, but 25 mM was required to kill EB. SR was inefficient for the used-to-solar-exposure Acanthamoeba strain. H2O2/SR improved the disinfection efficiency of treatments alone. 5 mM of H2O2/SR for 5 min eradicated both Acanthamoebae and EB, reducing up to 6 and 30 times the H2O2 dose and the SR fluence necessary, respectively. The Acanthamoeba strain that had already overcome water treatments was more resistant to all the treatments than the freshwater strain. This study underlines the protective role of amoebae in disinfection processes and the wide pathogenic microorganism spectrum that can overcome water treatments thanks to this “trojan horse”. More research is needed to optimize conditions and establish H2O2/SR as an efficient disinfection treatment that prevents waterborne and nosocomial infections of endosymbiont microorganisms according to water use.
Graphical Abstract
000132473 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B43-23R$$9info:eu-repo/grantAgreement/ES/MICINN/AEI/TED2021-129267B-I00
000132473 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000132473 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000132473 700__ $$aMarez, Carlissa
000132473 700__ $$0(orcid)0000-0002-9981-9045$$aChueca, Patricia$$uUniversidad de Zaragoza
000132473 700__ $$0(orcid)0000-0003-0765-7227$$aGoñi, Pilar$$uUniversidad de Zaragoza
000132473 700__ $$0(orcid)0000-0003-3014-0322$$aOrmad, Maria P.$$uUniversidad de Zaragoza
000132473 7102_ $$11011$$2660$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Parasitología
000132473 7102_ $$11001$$2X$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Técnica. Lab. y Talleres
000132473 7102_ $$15005$$2790$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Tecnologi. Medio Ambiente
000132473 773__ $$g431 (2024), 114562 [8 pp.]$$pCatal. today$$tCatalysis Today$$x0920-5861
000132473 8564_ $$s2095663$$uhttps://zaguan.unizar.es/record/132473/files/texto_completo.pdf$$yVersión publicada
000132473 8564_ $$s2505047$$uhttps://zaguan.unizar.es/record/132473/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000132473 909CO $$ooai:zaguan.unizar.es:132473$$particulos$$pdriver
000132473 951__ $$a2024-03-11-09:52:03
000132473 980__ $$aARTICLE