132478 20260217205508.0 doi 10.1039/d3lc01082a sideral 137637 ART-2024-137637 eng Olaizola-Rodrigo, Claudia Tuneable hydrogel patterns in pillarless microfluidic devices 2024 Access copy available to the general public Unrestricted Organ-on-chip (OOC) technology has recently emerged as a powerful tool to mimic physiological or pathophysiological conditions through cell culture in microfluidic devices. One of its main goals is bypassing animal testing and encouraging more personalized medicine. The recent incorporation of hydrogels as 3D scaffolds into microfluidic devices has changed biomedical research since they provide a biomimetic extracellular matrix to recreate tissue architectures. However, this technology presents some drawbacks such as the necessity for physical structures as pillars to confine these hydrogels, as well as the difficulty in reaching different shapes and patterns to create convoluted gradients or more realistic biological structures. In addition, pillars can also interfere with the fluid flow, altering the local shear forces and, therefore, modifying the mechanical environment in the OOC model. In this work, we present a methodology based on a plasma surface treatment that allows building cell culture chambers with abutment-free patterns capable of producing precise shear stress distributions. Therefore, pillarless devices with arbitrary geometries are needed to obtain more versatile, reliable, and biomimetic experimental models. Through computational simulation studies, these shear stress changes are demonstrated in different designed and fabricated geometries. To prove the versatility of this new technique, a blood–brain barrier model has been recreated, achieving an uninterrupted endothelial barrier that emulates part of the neurovascular network of the brain. Finally, we developed a new technology that could avoid the limitations mentioned above, allowing the development of biomimetic OOC models with complex and adaptable geometries, with cell-to-cell contact if required, and where fluid flow and shear stress conditions could be controlled. info:eu-repo/grantAgreement/ES/AEI/PID2021-126051OB-C41 info:eu-repo/grantAgreement/ES/DGA/T62-230R info:eu-repo/grantAgreement/EC/H2020/829010/EU/Advanced and versatile PRInting platform for the next generation of active Microfluidic dEvices/PRIME This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 829010-PRIME info:eu-repo/grantAgreement/ES/MCIU/RTI2018-097038-B-C21 info:eu-repo/grantAgreement/ES/MCIU/RTI2018-097038-B-C22 info:eu-repo/grantAgreement/ES/MICINN/PDC2022-133918-C21 info:eu-repo/grantAgreement/ES/MINECO/DIN2020-011544 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 5.4 2024 BIOCHEMICAL RESEARCH METHODS 8 / 86 = 0.093 2024 Q1 T1 CHEMISTRY, ANALYTICAL 20 / 111 = 0.18 2024 Q1 T1 INSTRUMENTS & INSTRUMENTATION 11 / 79 = 0.139 2024 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 62 / 239 = 0.259 2024 Q2 T1 NANOSCIENCE & NANOTECHNOLOGY 51 / 147 = 0.347 2024 Q2 T2 1.201 2024 Biochemistry 2024 Q1 Bioengineering 2024 Q1 Biomedical Engineering 2024 Q1 Chemistry (miscellaneous) 2024 Q1 Nanoscience and Nanotechnology 2024 Q2 10.8 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Palma-Florez, Sujey Randelovic, Teodora (orcid)0000-0001-7232-7588 Bayona, Clara (orcid)0000-0001-6786-2041 Ashrafi, Mehran Universidad de Zaragoza Samitier, Josep Lagunas, Anna Mir, Mònica Doblaré, Manuel Universidad de Zaragoza (orcid)0000-0001-8741-6452 Ochoa, Ignacio Universidad de Zaragoza (orcid)0000-0003-2410-5678 Monge, Rosa Oliván, Sara Universidad de Zaragoza (orcid)0000-0003-0156-4230 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 24, 7 (2024), 2094-2106 Lab chip Lab on a chip 1473-0197 2474546 http://zaguan.unizar.es/record/132478/files/texto_completo.pdf Versión publicada 2618562 http://zaguan.unizar.es/record/132478/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:132478 articulos driver 2026-02-17-20:24:08 ARTICLE