000132782 001__ 132782
000132782 005__ 20240315113107.0
000132782 0247_ $$2doi$$a10.1039/d3ja00378g
000132782 0248_ $$2sideral$$a137701
000132782 037__ $$aART-2024-137701
000132782 041__ $$aeng
000132782 100__ $$0(orcid)0000-0002-9181-2105$$aGimenez-Ingalaturre, Ana C.
000132782 245__ $$aPerformance of single-cell ICP-MS for quantitative biodistribution studies of silver interactions with bacteria
000132782 260__ $$c2024
000132782 5060_ $$aAccess copy available to the general public$$fUnrestricted
000132782 5203_ $$aSingle-cell analysis using inductively coupled plasma mass spectrometry (SC-ICP-MS) is an analytical methodology that allows to obtain qualitative and quantitative information of the element content of bioparticles (cells, bacteria, unicellular algae, yeasts…) on a cell-by-cell basis. In this study, two commercially available nebulisation systems for introduction of intact bioparticles were evaluated, showing similar performance. Bacteria (E. coli) exposed to Ag(I) and 10 nm Ag nanoparticles were detected and their complete silver content distributions successfully recorded. Analysis of the corresponding spheroplasts, obtained after enzymatic digestion of the bacterial outer membrane with lysozyme, allowed information about the intracellular silver to be obtained, providing an insight into the biodistribution of silver in the bacteria. Likewise, validity of the quantitative information obtained by SC-ICP-MS was evaluated by applying methodology based on the estimation of silver mass per bioparticle critical values and successive dilutions. The quantitative information obtained by SC-ICP-MS for the total content of silver in bacteria and spheroplasts was confirmed by acid digestion followed by conventional ICP-MS analysis. Bacteria exposed to silver nanoparticles accumulated lower amounts of silver than those exposed to ionic silver, the latter being totally internalized, showing the higher bioavailability of ionic silver. This study proves for the first time the suitability of SC-ICP-MS for the quantitative determination of silver in bacteria, as well as in spheroplasts when combined with lysozyme digestion. Limits of detection of 7 ag of Ag per bioparticle, 500 bioparticles per mL and 38 ng of total Ag per litre of bacteria/spheroplast suspension were achieved.
000132782 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FEDER/E29-23R$$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-123203OB-I00$$9info:eu-repo/grantAgreement/ES/NextGenerationEU/MZ-240621
000132782 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000132782 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000132782 700__ $$0(orcid)0000-0002-7931-3901$$aAbad-Álvaro, Isabel$$uUniversidad de Zaragoza
000132782 700__ $$0(orcid)0000-0003-0765-7227$$aGoñi, Pilar$$uUniversidad de Zaragoza
000132782 700__ $$aBillimoria, Kharmen
000132782 700__ $$aGoenaga-Infante, Heidi
000132782 700__ $$0(orcid)0000-0002-4169-0357$$aLaborda, Francisco$$uUniversidad de Zaragoza
000132782 7102_ $$11011$$2660$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Parasitología
000132782 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000132782 773__ $$g39, 3 (2024), 743-753$$pJ. anal. at. spectrom.$$tJournal of Analytical Atomic Spectrometry$$x0267-9477
000132782 8564_ $$s682302$$uhttps://zaguan.unizar.es/record/132782/files/texto_completo.pdf$$yVersión publicada
000132782 8564_ $$s2758411$$uhttps://zaguan.unizar.es/record/132782/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000132782 909CO $$ooai:zaguan.unizar.es:132782$$particulos$$pdriver
000132782 951__ $$a2024-03-15-08:49:28
000132782 980__ $$aARTICLE