132789 20260217205448.0 doi 10.1016/j.foodchem.2023.138296 sideral 137719 ART-2024-137719 eng Civera, Alba (orcid)0000-0001-7719-6388 Sensitive ELISA and lateral flow immunoassay for the detection of walnut traces in processed food and working surfaces 2024 Access copy available to the general public Unrestricted Walnut represents one of the most allergenic nuts that can be found as a hidden allergen. In this study, sandwich ELISA and lateral flow immunoassay (LFIA), based on the determination of Jug r 1, were developed to detect walnut. Cross-reactivity was only found with Pecan nut among a panel of 88 food ingredients tested. ELISA and LFIA could detect 0.25 and 0.5 µg/g of walnut protein in complex food matrices spiked with walnut extract, respectively. Furthermore, walnut was detected in blended (chocolate) and incurred foods (ice cream and bread) added with ground walnut at levels of 0.5 and 1.5 µg protein/g by ELISA and LFIA, respectively. LFIA could also detect 0.1 μg of walnut protein in working surfaces. ELISA displayed acceptable precision and high recovery (71–97 %) and both tests were robust. This study shows that developed ELISA and LFIA are reliable tools to be applied in allergen control programs. info:eu-repo/grantAgreement/ES/DGA/A20-23R info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 9.8 2024 CHEMISTRY, APPLIED 6 / 76 = 0.079 2024 Q1 T1 NUTRITION & DIETETICS 4 / 113 = 0.035 2024 Q1 T1 FOOD SCIENCE & TECHNOLOGY 7 / 182 = 0.038 2024 Q1 T1 1.952 2024 Analytical Chemistry 2024 Q1 Medicine (miscellaneous) 2024 Q1 Food Science 2024 Q1 18.3 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Esteban, Clara Universidad de Zaragoza (orcid)0009-0005-0336-7582 Mata, Luis Sánchez, Lourdes Universidad de Zaragoza (orcid)0000-0001-5964-823X Galan-Malo, Patricia Pérez, María D. Universidad de Zaragoza (orcid)0000-0003-2555-8425 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 441 (2024), 138296 [9 pp.] Food chem. Food Chemistry 0308-8146 512551 http://zaguan.unizar.es/record/132789/files/texto_completo.pdf Versión publicada 2544709 http://zaguan.unizar.es/record/132789/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:132789 articulos driver 2026-02-17-20:18:28 ARTICLE