000132791 001__ 132791
000132791 005__ 20240315113107.0
000132791 0247_ $$2doi$$a10.1021/acs.langmuir.7b01368
000132791 0248_ $$2sideral$$a129399
000132791 037__ $$aART-2017-129399
000132791 041__ $$aeng
000132791 100__ $$aKumar, Ravi
000132791 245__ $$aPolymer pen lithography with lipids for large-area gradient patterns
000132791 260__ $$c2017
000132791 5060_ $$aAccess copy available to the general public$$fUnrestricted
000132791 5203_ $$aGradient patterns comprising bioactive compounds over comparably (in regard to a cell size) large areas are key for many applications in the biomedical sector, in particular, for cell screening assays, guidance, and migration experiments. Polymer pen lithography (PPL) as an inherent highly parallel and large area technique has a great potential to serve in the fabrication of such patterns. We present strategies for the printing of functional phospholipid patterns via PPL that provide tunable feature size and feature density gradients over surface areas of several square millimeters. By controlling the printing parameters, two transfer modes can be achieved. Each of these modes leads to different feature morphologies. By increasing the force applied to the elastomeric pens, which increases the tip–surface contact area and boosts the ink delivery rate, a switch between a dip-pen nanolithography (DPN) and a microcontact printing (μCP) transfer mode can be induced. A careful inking procedure ensuring a homogeneous and not-too-high ink-load on the PPL stamp ensures a membrane-spreading dominated transfer mode, which, used in combination with smooth and hydrophilic substrates, generates features with constant height, independently of the applied force of the pens. Ultimately, this allows us to obtain a gradient of feature sizes over a mm2 substrate, all having the same height on the order of that of a biological cellular membrane. These strategies allow the construction of membrane structures by direct transfer of the lipid mixture to the substrate, without requiring previous substrate functionalization, in contrast to other molecular inks, where structure is directly determined by the printing process itself. The patterns are demonstrated to be viable for subsequent protein binding, therefore adding to a flexible feature library when gradients of protein presentation are desired.
000132791 536__ $$9info:eu-repo/grantAgreement/EC/FP7/328163/EU/In depth characterization of bio-mimetic lipid membrane structures generated by dip-pen nanolithography/DPNLipidMembranes
000132791 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000132791 590__ $$a3.789$$b2017
000132791 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b61 / 283 = 0.216$$c2017$$dQ1$$eT1
000132791 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b50 / 169 = 0.296$$c2017$$dQ2$$eT1
000132791 591__ $$aCHEMISTRY, PHYSICAL$$b48 / 144 = 0.333$$c2017$$dQ2$$eT2
000132791 592__ $$a1.479$$b2017
000132791 593__ $$aCondensed Matter Physics$$c2017$$dQ1
000132791 593__ $$aElectrochemistry$$c2017$$dQ1
000132791 593__ $$aSurfaces and Interfaces$$c2017$$dQ1
000132791 593__ $$aMedicine (miscellaneous)$$c2017$$dQ1
000132791 593__ $$aSpectroscopy$$c2017$$dQ1
000132791 593__ $$aMaterials Science (miscellaneous)$$c2017$$dQ1
000132791 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000132791 700__ $$0(orcid)0000-0002-8424-9780$$aUrtizberea, Ainhoa
000132791 700__ $$aGhosh, Souvik
000132791 700__ $$aBog, Uwe
000132791 700__ $$aRainer, Quinn
000132791 700__ $$aLenhert, Steven
000132791 700__ $$aFuchs, Harald
000132791 700__ $$aHirtz, Michael
000132791 773__ $$g33, 35 (2017), 8739-8748$$pLangmuir$$tLangmuir$$x0743-7463
000132791 8564_ $$s976671$$uhttps://zaguan.unizar.es/record/132791/files/texto_completo.pdf$$yPostprint
000132791 8564_ $$s2469861$$uhttps://zaguan.unizar.es/record/132791/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000132791 909CO $$ooai:zaguan.unizar.es:132791$$particulos$$pdriver
000132791 951__ $$a2024-03-15-08:49:46
000132791 980__ $$aARTICLE