133116 20260217205508.0 doi 10.1038/s41598-024-54051-7 sideral 137852 ART-2024-137852 eng Ramirez-Labrada, Ariel (orcid)0000-0002-3888-7036 Multiparametric in vitro and in vivo analysis of the safety profile of self-assembling peptides 2024 Access copy available to the general public Unrestricted Self-assembling peptides (SAPs) have gained significant attention in biomedicine because of their unique properties and ability to undergo molecular self-assembly driven by non-covalent interactions. By manipulating their composition and structure, SAPs can form well-ordered nanostructures with enhanced selectivity, stability and biocompatibility. SAPs offer advantages such as high chemical and biological diversity and the potential for functionalization. However, studies concerning its potentially toxic effects are very scarce, a limitation that compromises its potential translation to humans. This study investigates the potentially toxic effects of six different SAP formulations composed of natural amino acids designed for nervous tissue engineering and amenable to ready cross-linking boosting their biomechanical properties. All methods were performed in accordance with the relevant guidelines and regulations. A wound-healing assay was performed to evaluate how SAPs modify cell migration. The results in vitro demonstrated that SAPs did not induce genotoxicity neither skin sensitization. In vivo, SAPs were well-tolerated without any signs of acute systemic toxicity. Interestingly, SAPs were found to promote the migration of endothelial, macrophage, fibroblast, and neuronal-like cells in vitro, supporting a high potential for tissue regeneration. These findings contribute to the development and translation of SAP-based biomaterials for biomedical applications. info:eu-repo/grantAgreement/ES/AEI/PID2020-113963RB-I00 info:eu-repo/grantAgreement/ES/AEI/RYC2022-036627-I info:eu-repo/grantAgreement/ES/DGA/B29-23R info:eu-repo/grantAgreement/ES/DGA/LMP139-21 info:eu-repo/grantAgreement/ES/ISCIII/CB21-13-00087 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 3.9 2024 MULTIDISCIPLINARY SCIENCES 25 / 136 = 0.184 2024 Q1 T1 0.874 2024 Multidisciplinary 2024 Q1 6.7 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Santiago, Llipsy Pesini, Cecilia Arrieta, Marta Arias, Maykel (orcid)0000-0002-9730-2210 Calvo Pérez, Adanays Ciulla, Maria Gessica Forouharshad, Mahdi Pardo, Julian Universidad de Zaragoza (orcid)0000-0003-0154-0730 Gálvez, Eva M. Gelain, Fabrizio 1011 566 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Inmunología 14, 1 (2024), 4395 [19 pp.] Sci. rep. (Nat. Publ. Group) Scientific reports (Nature Publishing Group) 2045-2322 7274382 http://zaguan.unizar.es/record/133116/files/texto_completo.pdf Versión publicada 2111988 http://zaguan.unizar.es/record/133116/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:133116 articulos driver 2026-02-17-20:24:17 ARTICLE