000133152 001__ 133152
000133152 005__ 20240322124130.0
000133152 0247_ $$2doi$$a10.1016/j.rvsc.2024.105206
000133152 0248_ $$2sideral$$a137845
000133152 037__ $$aART-2024-137845
000133152 041__ $$aeng
000133152 100__ $$aGónzález, Mikel A.
000133152 245__ $$aSurveillance and screening of Stomoxyinae flies from Mallorca Island (Spain) reveal the absence of selected pathogens but confirm the presence of the endosymbiotic bacterium Wolbachia pipientis
000133152 260__ $$c2024
000133152 5060_ $$aAccess copy available to the general public$$fUnrestricted
000133152 5203_ $$aAdult brachycera biting flies can significantly impact livestock through both direct effects (reduction of food intake, disturbance, painful bites, and blood loss) and indirect effects (pathogen transmission), leading to substantial economic losses and production damage. This study aimed to assess the presence of blood-sucking flies in six mixed-animal farm environments on the island of Mallorca (Balearic Islands, Spain) by employing multiple trapping methods. Additionally, distribution maps of brachycera biting fly species recorded in Spain were created, based on data extracted thorough review of scientific literature and citizen digital databases. Investigation of several pathogens, including equine infectious anemia virus (EIAV), Anaplasmataceae bacteria, and piroplasm protozoa, was carried out using different PCR targets (18S rRNA, 16S rRNA, groESL, and tat genes). Citizen science databases and literature review corroborated the consistent distribution trend for two Stomoxyinae species, underscoring the importance of citizen collaboration as a complement to traditional entomological surveillance. Our study confirmed the presence of two biting Stomoxyinae species: the prevalent stable fly Stomoxys calcitrans across all sampled farms, and the horn fly Haematobia irritans, which turned out to be less abundant. DNA barcoding techniques validated the identification of the two species. Neither EIAV nor bacterial/protozoan pathogens were detected using the selected PCR targets in either fly species. However, Wolbachia pipientis (clustered in the supergroup A together with the only sequence of W. pipientis from the USA) was identified through PCR targeting 16S rRNA, groESL and wsp genes in all pools of H. irritans (n = 13) collected from two of the examined farms. This study represents the first attempt to investigate pathogens in Stomoxyinae biting flies in Spain. The discovery of the endosymbiotic Wolbachia organism in H. irritans represents the first record in Spain and the second from Europe. This finding holds significant implications for future research on the applications of this bacterium in biocontrol programs.
000133152 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000133152 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000133152 700__ $$0(orcid)0000-0001-8198-8118$$aRuiz-Arrondo, Ignacio$$uUniversidad de Zaragoza
000133152 700__ $$aBravo-Barriga, Daniel
000133152 700__ $$aCervera-Acedo, Cristina
000133152 700__ $$aSantibáñez, Paula
000133152 700__ $$aOteo, José A.
000133152 700__ $$aMiranda, Miguel A.
000133152 700__ $$aBarceló, Carlos
000133152 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000133152 773__ $$g171 (2024), e105206 [10 pp.]$$pRes. Vet. Sci.$$tRESEARCH IN VETERINARY SCIENCE$$x0034-5288
000133152 8564_ $$s3623053$$uhttps://zaguan.unizar.es/record/133152/files/texto_completo.pdf$$yVersión publicada
000133152 8564_ $$s2427122$$uhttps://zaguan.unizar.es/record/133152/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000133152 909CO $$ooai:zaguan.unizar.es:133152$$particulos$$pdriver
000133152 951__ $$a2024-03-22-09:48:09
000133152 980__ $$aARTICLE