000133154 001__ 133154
000133154 005__ 20240322124130.0
000133154 0247_ $$2doi$$a10.1371/journal.pone.0293547
000133154 0248_ $$2sideral$$a137842
000133154 037__ $$aART-2023-137842
000133154 041__ $$aeng
000133154 100__ $$0(orcid)0000-0001-8198-8118$$aRuiz-Arrondo, Ignacio$$uUniversidad de Zaragoza
000133154 245__ $$aIntegrated taxonomy of black flies (Diptera: Simuliidae) reveals unexpected diversity in the most arid ecosystem of Europe
000133154 260__ $$c2023
000133154 5060_ $$aAccess copy available to the general public$$fUnrestricted
000133154 5203_ $$aThe family Simuliidae includes more than 2000 species of black flies worldwide. Their morphological uniformity creates difficulty for species identification, which limits our knowledge of their ecology and vectorial role. We investigated the systematics of black flies in a semi-arid area of the Iberian Peninsula, an ecologically harsh environment for these organisms. Sampling adult black flies in three different habitats (by means of CDC traps) and in avian nest boxes and collecting immature stages in high-salinity rills provided a representative sample of the component species. A combination of approaches, including morphological, chromosomal, and molecular (based on the mitochondrial cytochrome C oxidase subunit I (COI) and internal transcribed spacer 2 (ITS2) genes) revealed five species: four common species (Simulium intermedium, S. petricolum, S. pseudequinum, and S. rubzovianum) and the first European record for S. mellah. Barcoding gap and phylogenetic analyses revealed that ITS2 is a key marker to identify the species, whereas the COI marker does not provide enough resolution to identify some species or infer their phylogenetic relationships. Morphological and chromosomal features are also provided to identify S. mellah unequivocally. Our study highlights the need for integrated studies of black flies in ecologically extreme habitats to increase our knowledge of their distribution, ecology, and potential risks for public health.
000133154 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/CGL2014-55969$$9info:eu-repo/grantAgreement/ES/MICINN/PGC2018-097426-B-C22
000133154 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000133154 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000133154 700__ $$aVeiga, Jesús
000133154 700__ $$aAdler, Peter
000133154 700__ $$aCollantes, Francisco
000133154 700__ $$aOteo, José A.
000133154 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000133154 773__ $$g18, 11 (2023), e0293547 [23 pp.]$$pPLoS One$$tPLoS ONE$$x1932-6203
000133154 8564_ $$s4202965$$uhttps://zaguan.unizar.es/record/133154/files/texto_completo.pdf$$yVersión publicada
000133154 8564_ $$s2380846$$uhttps://zaguan.unizar.es/record/133154/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000133154 909CO $$ooai:zaguan.unizar.es:133154$$particulos$$pdriver
000133154 951__ $$a2024-03-22-09:48:11
000133154 980__ $$aARTICLE