133170 20260217205516.0 doi 10.1016/j.idairyj.2024.105899 sideral 137811 ART-2024-137811 eng Abad, I. Universidad de Zaragoza Gastrointestinal digestion and technological treatments modify the antibacterial activity of lactoferrin supplemented dairy matrices against Staphylococcus aureus 2024 Access copy available to the general public Unrestricted Milk contains antimicrobial proteins, such as lactoferrin from whey or proteins from the milk fat globule membrane (MFGM), which can be used in functional foods to strengthen children and adult defenses. Foodborne bacteria, such as Staphylococcus aureus, can contaminate dairy products causing intoxication. The aim of this study was to evaluate the antibacterial activity of lactoferrin, free and in dairy matrices, against S. aureus before and after gastrointestinal digestion. Six dairy formulas, supplemented with lactoferrin and MFGM, were subjected to technological treatments and their antibacterial effect was analyzed after in vitro digestion. Intact lactoferrin slightly reduced S. aureus growth, but its digests lost this activity. Gastric digests of non-treated or homogenized formulas reduced significantly the bacterial growth, probably due to the antimicrobial peptides generated by pepsin, while pasteurization decreased such activity. Intestinal digests showed the greatest antibacterial effect, probably due to the action of intestinal enzymes and the generated peptides. info:eu-repo/grantAgreement/ES/DGA/A20-23R info:eu-repo/grantAgreement/ES/MICINN/PID2022-139104OB-I00 info:eu-repo/grantAgreement/ES/MINECO/AGL2017-82987 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 3.4 2024 FOOD SCIENCE & TECHNOLOGY 73 / 182 = 0.401 2024 Q2 T2 0.849 2024 Food Science 2024 Q1 Applied Microbiology and Biotechnology 2024 Q2 7.4 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Bailac, A. Pérez, M.D. Universidad de Zaragoza (orcid)0000-0003-2555-8425 Carramiñana, J.J. Universidad de Zaragoza (orcid)0000-0002-3320-9295 Calvo, M. Universidad de Zaragoza (orcid)0000-0001-5373-9210 Sánchez, L. 2008 640 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Nutrición Bromatología 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 153 (2024), 105899 [8 pp.] Int. dairy j. INTERNATIONAL DAIRY JOURNAL 0958-6946 611862 http://zaguan.unizar.es/record/133170/files/texto_completo.pdf Versión publicada 2702702 http://zaguan.unizar.es/record/133170/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:133170 articulos driver 2026-02-17-20:26:53 ARTICLE