000133281 001__ 133281
000133281 005__ 20240410085329.0
000133281 0247_ $$2doi$$a10.3390/vetsci11030103
000133281 0248_ $$2sideral$$a138046
000133281 037__ $$aART-2024-138046
000133281 041__ $$aeng
000133281 100__ $$0(orcid)0000-0001-5750-3643$$aPeris, María Paz$$uUniversidad de Zaragoza
000133281 245__ $$aIdentification of sarcocystis spp. in slaughtered sheep from Spain and evaluation of bradyzoite viability after freezing
000133281 260__ $$c2024
000133281 5060_ $$aAccess copy available to the general public$$fUnrestricted
000133281 5203_ $$aSarcocystis spp. are complex apicomplexan parasites that cause a substantial economic impact on livestock used for meat production. These parasites are present worldwide. Our study aimed to identify Sarcocystis species affecting sheep meat in southern–central Spain and to evaluate the effectiveness of freezing for parasite inactivation. A total of 210 condemned samples of sheep meat were thoroughly assessed grossly and microscopically; the presence of macro- and microcysts was confirmed. The samples were then frozen at −20 °C for various time intervals (24, 48, 72, 96, 120, and 144 h) and compared with untreated samples. Bradyzoites were isolated through pepsin digestion for subsequent molecular analysis and viability assessment, employing trypan blue and double fluorescence staining techniques. Our measurements confirmed the presence of S. tenella, S. gigantea, and S. medusiformis in Spanish domestic sheep. Freezing for 96 to 144 h resulted in a significant reduction in parasite viability, with a robust correlation observed between the two staining methods. Both stains effectively measured the viability of Sarcocystis, thereby promising future advances in meat safety.
000133281 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A05-17R$$9info:eu-repo/grantAgreement/ES/MICINN/RTC-2017-6594-2
000133281 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000133281 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000133281 700__ $$aGracia, María Jesús
000133281 700__ $$0(orcid)0000-0001-6629-6842$$aMoreno, Bernardino$$uUniversidad de Zaragoza
000133281 700__ $$aJuan-Puente, Paula
000133281 700__ $$0(orcid)0000-0001-9778-6545$$aMorales, Mariano$$uUniversidad de Zaragoza
000133281 700__ $$aSerrano, María
000133281 700__ $$aManzano, María Dolores
000133281 700__ $$0(orcid)0000-0002-4121-8087$$aHalaihel, Nabil$$uUniversidad de Zaragoza
000133281 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, Juan$$uUniversidad de Zaragoza
000133281 700__ $$0(orcid)0000-0002-2048-4749$$aCastillo, Juan Antonio$$uUniversidad de Zaragoza
000133281 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000133281 773__ $$g11, 3 (2024), 103$$tVeterinary Sciences$$x2306-7381
000133281 8564_ $$s1671309$$uhttps://zaguan.unizar.es/record/133281/files/texto_completo.pdf$$yVersión publicada
000133281 8564_ $$s2733393$$uhttps://zaguan.unizar.es/record/133281/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000133281 909CO $$ooai:zaguan.unizar.es:133281$$particulos$$pdriver
000133281 951__ $$a2024-04-10-08:38:33
000133281 980__ $$aARTICLE