000133297 001__ 133297
000133297 005__ 20241220133820.0
000133297 0247_ $$2doi$$a10.1111/andr.13624
000133297 0248_ $$2sideral$$a137946
000133297 037__ $$aART-2024-137946
000133297 041__ $$aeng
000133297 100__ $$0(orcid)0000-0001-9802-8199$$aLópez-Royo, Tresa$$uUniversidad de Zaragoza
000133297 245__ $$aSex differences on constitutive long non-coding RNA expression: Modulatory effect of estradiol and testosterone in muscle cells
000133297 260__ $$c2024
000133297 5060_ $$aAccess copy available to the general public$$fUnrestricted
000133297 5203_ $$aAbstractIntroductionDespite the growing awareness of sexual dimorphism between males and females under pathological and physiological conditions, sex bias in biomedical research in animal models and patients is still present nowadays. The main objective of this work was to investigate sex differences in constitutive long non‐coding RNA expression in spinal cord and skeletal muscle from wild‐type mice.Materials and MethodsTo assess the influence of gender on long non‐coding RNAs, we extracted RNA from tissues of male and female mice and analyzed the expression on nine long non‐coding RNAs, selected for being among the most commonly studied or exerting an important role in muscle, at 50, 60, and 120 days of age.Results and DiscussionWe observed age‐ and tissue‐dependent significant sex differences, being more prominent in skeletal muscle. We also studied the effect of sex steroid hormones on long non‐coding RNA expression in vitro, noticing a modulation of long non‐coding RNA levels upon estradiol and dihydrotestosterone treatment in muscle.ConclusionsTaken together, results obtained evidenced sex differences on constitutive long non‐coding RNA expression and suggested an influence of steroid hormones complementary to other possible factors. These findings emphasize the importance of including both sexes in experimental design to minimize any potential sex bias.
000133297 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/FEDER/PI21-00372
000133297 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000133297 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000133297 700__ $$0(orcid)0000-0002-7277-4318$$aMoreno-Martínez, Laura$$uUniversidad de Zaragoza
000133297 700__ $$0(orcid)0000-0003-2436-7205$$aMoreno-García, Leticia
000133297 700__ $$0(orcid)0000-0001-5193-7782$$aCalvo, Ana Cristina$$uUniversidad de Zaragoza
000133297 700__ $$0(orcid)0000-0002-7477-8742$$aManzano, Raquel$$uUniversidad de Zaragoza
000133297 700__ $$0(orcid)0000-0001-5687-6704$$aOsta, Rosario$$uUniversidad de Zaragoza
000133297 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000133297 7102_ $$11012$$2315$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Farmacología
000133297 773__ $$g12, 8 (2024), 1887-1896$$pAndrology$$tAndrology$$x2047-2919
000133297 8564_ $$s1702202$$uhttps://zaguan.unizar.es/record/133297/files/texto_completo.pdf$$yVersión publicada
000133297 8564_ $$s2364022$$uhttps://zaguan.unizar.es/record/133297/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000133297 909CO $$ooai:zaguan.unizar.es:133297$$particulos$$pdriver
000133297 951__ $$a2024-12-20-13:37:43
000133297 980__ $$aARTICLE