000133311 001__ 133311
000133311 005__ 20240410085329.0
000133311 0247_ $$2doi$$a10.1039/d3tb02640j
000133311 0248_ $$2sideral$$a138021
000133311 037__ $$aART-2024-138021
000133311 041__ $$aeng
000133311 100__ $$0(orcid)0000-0002-1647-8207$$aPamplona, Regina
000133311 245__ $$aEvaluation of gelatin-based hydrogels for colon and pancreas studies using 3D in vitro cell culture
000133311 260__ $$c2024
000133311 5060_ $$aAccess copy available to the general public$$fUnrestricted
000133311 5203_ $$aBiomimetic 3D models emerged some decades ago to address 2D cell culture limitations in the field of replicating biological phenomena, structures or functions found in nature. The fabrication of hydrogels for cancer disease research enables the study of cell processes including growth, proliferation and migration and their 3D design is based on the encapsulation of tumoral cells within a tunable matrix. In this work, a platform of gelatin methacrylamide (GelMA)-based photocrosslinked scaffolds with embedded colorectal (HCT-116) or pancreatic (MIA PaCa-2) cancer cells is presented. Prior to cell culture, the mechanical characterization of hydrogels was assessed in terms of stiffness and swelling behavior. Modifications of the UV curing time enabled a fine tuning of the mechanical properties, which at the same time, showed susceptibility to the chemical composition and crosslinking mechanism. All scaffolds displayed excellent cytocompatibility with both tumoral cells while eliciting various cell responses depending on the microenvironment features. Individual and collective cell migration were observed for HCT-116 and MIA PaCa-2 cell lines, highlighting the ability of the colorectal cancer cells to cluster into aggregates of different sizes governed by the surrounding matrix. Additionally, metabolic activity results pointed out to the development of a more proliferative phenotype within stiffer networks. These findings confirm the suitability of the presented platform of GelMA-based hydrogels to conduct 3D cell culture experiments and explore biological processes associated with colorectal and pancreatic cancer.
000133311 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E15-23R$$9info:eu-repo/grantAgreement/ES/ISCIII/CB06-01/00263$$9info:eu-repo/grantAgreement/ES/MICINN/PID2020-118485RB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2022-141276OB-I00$$9info:eu-repo/grantAgreement/ES/MINECO/DI2017-09585
000133311 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000133311 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000133311 700__ $$aGonzález-Lana, Sandra
000133311 700__ $$0(orcid)0000-0003-2410-5678$$aOchoa, Ignacio$$uUniversidad de Zaragoza
000133311 700__ $$0(orcid)0000-0003-0702-8260$$aMartín-Rapún, Rafael$$uUniversidad de Zaragoza
000133311 700__ $$0(orcid)0000-0003-3900-2866$$aSánchez-Somolinos, Carlos
000133311 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000133311 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000133311 773__ $$g12 (2024), 3144-3160$$pJ. mater. chem. B$$tJournal of Materials Chemistry B$$x2050-750X
000133311 8564_ $$s9083461$$uhttps://zaguan.unizar.es/record/133311/files/texto_completo.pdf$$yVersión publicada
000133311 8564_ $$s2617552$$uhttps://zaguan.unizar.es/record/133311/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000133311 909CO $$ooai:zaguan.unizar.es:133311$$particulos$$pdriver
000133311 951__ $$a2024-04-10-08:39:15
000133311 980__ $$aARTICLE