133311 20260217205515.0 doi 10.1039/d3tb02640j sideral 138021 ART-2024-138021 eng Pamplona, Regina (orcid)0000-0002-1647-8207 Evaluation of gelatin-based hydrogels for colon and pancreas studies using 3D in vitro cell culture 2024 Access copy available to the general public Unrestricted Biomimetic 3D models emerged some decades ago to address 2D cell culture limitations in the field of replicating biological phenomena, structures or functions found in nature. The fabrication of hydrogels for cancer disease research enables the study of cell processes including growth, proliferation and migration and their 3D design is based on the encapsulation of tumoral cells within a tunable matrix. In this work, a platform of gelatin methacrylamide (GelMA)-based photocrosslinked scaffolds with embedded colorectal (HCT-116) or pancreatic (MIA PaCa-2) cancer cells is presented. Prior to cell culture, the mechanical characterization of hydrogels was assessed in terms of stiffness and swelling behavior. Modifications of the UV curing time enabled a fine tuning of the mechanical properties, which at the same time, showed susceptibility to the chemical composition and crosslinking mechanism. All scaffolds displayed excellent cytocompatibility with both tumoral cells while eliciting various cell responses depending on the microenvironment features. Individual and collective cell migration were observed for HCT-116 and MIA PaCa-2 cell lines, highlighting the ability of the colorectal cancer cells to cluster into aggregates of different sizes governed by the surrounding matrix. Additionally, metabolic activity results pointed out to the development of a more proliferative phenotype within stiffer networks. These findings confirm the suitability of the presented platform of GelMA-based hydrogels to conduct 3D cell culture experiments and explore biological processes associated with colorectal and pancreatic cancer. info:eu-repo/grantAgreement/ES/DGA/E15-23R info:eu-repo/grantAgreement/ES/ISCIII/CB06-01/00263 info:eu-repo/grantAgreement/ES/MICINN/PID2020-118485RB-I00 info:eu-repo/grantAgreement/ES/MICINN/PID2022-141276OB-I00 info:eu-repo/grantAgreement/ES/MINECO/DI2017-09585 info:eu-repo/semantics/openAccess by-nc https://creativecommons.org/licenses/by-nc/4.0/deed.es 5.8 2024 MATERIALS SCIENCE, BIOMATERIALS 16 / 55 = 0.291 2024 Q2 T1 1.159 2024 Biomedical Engineering 2024 Q1 Medicine (miscellaneous) 2024 Q1 Materials Science (miscellaneous) 2024 Q1 Chemistry (miscellaneous) 2024 Q1 10.4 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion González-Lana, Sandra Ochoa, Ignacio Universidad de Zaragoza (orcid)0000-0003-2410-5678 Martín-Rapún, Rafael Universidad de Zaragoza (orcid)0000-0003-0702-8260 Sánchez-Somolinos, Carlos (orcid)0000-0003-3900-2866 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 2013 765 Universidad de Zaragoza Dpto. Química Orgánica Área Química Orgánica 12 (2024), 3144-3160 J. mater. chem. B Journal of Materials Chemistry B 2050-750X 9083461 http://zaguan.unizar.es/record/133311/files/texto_completo.pdf Versión publicada 2617552 http://zaguan.unizar.es/record/133311/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:133311 articulos driver 2026-02-17-20:26:41 ARTICLE