000133352 001__ 133352
000133352 005__ 20240412150654.0
000133352 0247_ $$2doi$$a10.1016/j.heliyon.2024.e28566
000133352 0248_ $$2sideral$$a138084
000133352 037__ $$aART-2024-138084
000133352 041__ $$aeng
000133352 100__ $$aAlonso Llorente, Alba
000133352 245__ $$aGenetic polymorphisms of CYP2C19 in ecuadorian population: An interethnic approach
000133352 260__ $$c2024
000133352 5060_ $$aAccess copy available to the general public$$fUnrestricted
000133352 5203_ $$aIntroduction: CYP2C19 is a highly polymorphic gene responsible for metabolizing commonly used drugs. CYP2C19*2,*3 (loss of activity alleles) and *17 (increased activity allele) are the principal alleles included in clinical guidelines, however their prevalence varies among different ethnicities. Ecuadorian population is formed by Mestizos, Afrodescendants and Native Americans and frequency of CYP2C19 alleles could be different among them. The objective of this study was to establish the frequency of these variants in the different populations of Ecuador and to compare them with other populations. Materials and methods: DNA from 105 Afrodescendants, 75 Native Americans of the Kichwa ethnicity, and 33 Mestizos Ecuadorians was analyzed by nested-PCR to identify CYP2C19*17 carriers. CYP2C19*2 allele was analyzed in DNA from 78 Afrodescendants, 29 Native Americans of the Kichwa, and 16 Mestizos by TaqMan Allelic Discrimination Assay. CYP2C19*3 was analyzed in 33 Afrodescendants by nested-PCR. Results: The global frequencies of the alternate alleles were 14.22% (CYP2C19*2) and 2.10% (CYP2C19*17). No differences (p > 0.05) were observed among the subgroups. No CYP2C19*3 carrier was identified. CYP2C19*2 frequencies in Ecuador were similar to the ones reported in Europe, Africa and Middle East countries and to some American populations. Low CYP2C19*17 frequencies, like the ones in our population, were also observed in East and South Asia and in Native American groups. Discussion: Absence of differences in the ethnic groups in Ecuador for CYP2C19*2 and *17 could be due to either a bias in sample selection (ethnic group was assed by self-identification) or to a high interethnic admixture in the Ecuadorian population that would had diluted genetic differences. In addition, CYP2C19*2, *3, and *17 alleles frequencies in our study suggest that Ecuadorians ancestry is mostly of Native American origin.
000133352 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000133352 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000133352 700__ $$aSalgado Garrido, Josefa
000133352 700__ $$aTeijido Hermida, Óscar
000133352 700__ $$aGonzález Andrade, Fabricio
000133352 700__ $$aValiente Martín, Alberto
000133352 700__ $$0(orcid)0000-0001-8064-8138$$aFanlo Villacampa, Ana Julia$$uUniversidad de Zaragoza
000133352 700__ $$0(orcid)0000-0003-4629-6743$$aVicente Romero, Jorge$$uUniversidad de Zaragoza
000133352 7102_ $$11012$$2315$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Farmacología
000133352 773__ $$g10, 7 (2024), e28566 [12 pp.]$$pHeliyon$$tHeliyon$$x2405-8440
000133352 8564_ $$s542189$$uhttps://zaguan.unizar.es/record/133352/files/texto_completo.pdf$$yVersión publicada
000133352 8564_ $$s1820623$$uhttps://zaguan.unizar.es/record/133352/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000133352 909CO $$ooai:zaguan.unizar.es:133352$$particulos$$pdriver
000133352 951__ $$a2024-04-12-13:58:33
000133352 980__ $$aARTICLE