000133363 001__ 133363
000133363 005__ 20240412150654.0
000133363 0247_ $$2doi$$a10.1016/j.smallrumres.2024.107248
000133363 0248_ $$2sideral$$a138056
000133363 037__ $$aART-2024-138056
000133363 041__ $$aeng
000133363 100__ $$0(orcid)0000-0002-7822-6646$$aLacasta, Delia$$uUniversidad de Zaragoza
000133363 245__ $$aComparative study of three different routes of experimental inoculation of the orf virus
000133363 260__ $$c2024
000133363 5060_ $$aAccess copy available to the general public$$fUnrestricted
000133363 5203_ $$aThree different methods of inducing experimental infection of lambs with orf virus (ORFV), the cause of Contagious Ecthyma, were examined in nine animals; intradermal inoculation, subcutaneous injection and epithelial scarification. The objective was to identify the most appropriate experimental method to reproduce the disease with lesions of similar severity in all infected animals. Subcutaneous inoculation failed to reproduce orf lesions in two of the three infected animals, whereas both the groups that were inoculated by intradermal and scarification routes, respectively, displayed a significantly higher number of lesions at 12 dpi than the group inoculated subcutaneously. However, the lesions following scarification spread from the inoculation site with no ORFV-associated lesions found in other areas of the mucous membrane or skin. Finally, following intradermal inoculation, ORFV-associated lesions developed homogeneously in all infected animals, with lesions progressing from the point of inoculation in different areas of the skin of the lips, yet also spreading to the interior of the mouth, gums, palate and tongue, as occurs in natural infections. Thus, it was concluded that for studies investigating the efficacy of new approaches to treatment and vaccination for improved welfare of affected animals and control of ORFV transmission, the most appropriate route for experimental ORFV infection is intradermal inoculation
000133363 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A15-17R$$9info:eu-repo/grantAgreement/ES/DGA-FSE/Construyendo Aragón 2016–2020-A15-17R
000133363 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000133363 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000133363 700__ $$aCuadra, María
000133363 700__ $$aGómez, Alex$$uUniversidad de Zaragoza
000133363 700__ $$aOrtín, Aurora
000133363 700__ $$0(orcid)0000-0002-7655-2472$$aRuiz de Arcaute, Marta$$uUniversidad de Zaragoza
000133363 700__ $$0(orcid)0000-0002-2724-2785$$aRamos, Juan José$$uUniversidad de Zaragoza
000133363 700__ $$0(orcid)0000-0001-6209-4282$$aVillanueva-Saz, Sergio$$uUniversidad de Zaragoza
000133363 700__ $$0(orcid)0000-0001-5026-5144$$aTejedor, M. Teresa$$uUniversidad de Zaragoza
000133363 700__ $$0(orcid)0000-0002-8474-2831$$aRuiz, Héctor$$uUniversidad de Zaragoza
000133363 700__ $$0(orcid)0000-0003-2957-1379$$aVerde, Maite$$uUniversidad de Zaragoza
000133363 700__ $$aReina, Ramsés
000133363 700__ $$0(orcid)0000-0002-4557-6033$$aNavarro, Teresa$$uUniversidad de Zaragoza
000133363 700__ $$aQuilez, Pablo$$uUniversidad de Zaragoza
000133363 700__ $$0(orcid)0000-0002-2685-1730$$aRodríguez-Largo, Ana
000133363 700__ $$aWindsor, Peter Andrew
000133363 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000133363 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000133363 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000133363 773__ $$g233 (2024), 107248 [9 pp.]$$pSmall rumin. res.$$tSmall Ruminant Research$$x0921-4488
000133363 8564_ $$s8332007$$uhttps://zaguan.unizar.es/record/133363/files/texto_completo.pdf$$yVersión publicada
000133363 8564_ $$s2491382$$uhttps://zaguan.unizar.es/record/133363/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000133363 909CO $$ooai:zaguan.unizar.es:133363$$particulos$$pdriver
000133363 951__ $$a2024-04-12-13:58:45
000133363 980__ $$aARTICLE