133363 20260217205501.0 doi 10.1016/j.smallrumres.2024.107248 sideral 138056 ART-2024-138056 eng Lacasta, Delia Universidad de Zaragoza (orcid)0000-0002-7822-6646 Comparative study of three different routes of experimental inoculation of the orf virus 2024 Access copy available to the general public Unrestricted Three different methods of inducing experimental infection of lambs with orf virus (ORFV), the cause of Contagious Ecthyma, were examined in nine animals; intradermal inoculation, subcutaneous injection and epithelial scarification. The objective was to identify the most appropriate experimental method to reproduce the disease with lesions of similar severity in all infected animals. Subcutaneous inoculation failed to reproduce orf lesions in two of the three infected animals, whereas both the groups that were inoculated by intradermal and scarification routes, respectively, displayed a significantly higher number of lesions at 12 dpi than the group inoculated subcutaneously. However, the lesions following scarification spread from the inoculation site with no ORFV-associated lesions found in other areas of the mucous membrane or skin. Finally, following intradermal inoculation, ORFV-associated lesions developed homogeneously in all infected animals, with lesions progressing from the point of inoculation in different areas of the skin of the lips, yet also spreading to the interior of the mouth, gums, palate and tongue, as occurs in natural infections. Thus, it was concluded that for studies investigating the efficacy of new approaches to treatment and vaccination for improved welfare of affected animals and control of ORFV transmission, the most appropriate route for experimental ORFV infection is intradermal inoculation info:eu-repo/grantAgreement/ES/DGA/A15-17R info:eu-repo/semantics/openAccess by-nc https://creativecommons.org/licenses/by-nc/4.0/deed.es 1.4 2024 AGRICULTURE, DAIRY & ANIMAL SCIENCE 46 / 86 = 0.535 2024 Q3 T2 0.439 2024 Food Animals 2024 Q2 Animal Science and Zoology 2024 Q2 3.1 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Cuadra, María Gómez, Alex Universidad de Zaragoza (orcid)0000-0002-9723-9004 Ortín, Aurora Ruiz de Arcaute, Marta Universidad de Zaragoza (orcid)0000-0002-7655-2472 Ramos, Juan José Universidad de Zaragoza (orcid)0000-0002-2724-2785 Villanueva-Saz, Sergio Universidad de Zaragoza (orcid)0000-0001-6209-4282 Tejedor, M. Teresa Universidad de Zaragoza (orcid)0000-0001-5026-5144 Ruiz, Héctor Universidad de Zaragoza (orcid)0000-0002-8474-2831 Verde, Maite Universidad de Zaragoza (orcid)0000-0003-2957-1379 Reina, Ramsés Navarro, Teresa Universidad de Zaragoza (orcid)0000-0002-4557-6033 Quilez, Pablo Universidad de Zaragoza (orcid)0009-0003-9757-3840 Rodríguez-Largo, Ana (orcid)0000-0002-2685-1730 Windsor, Peter Andrew 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 233 (2024), 107248 [9 pp.] Small rumin. res. Small Ruminant Research 0921-4488 8332007 http://zaguan.unizar.es/record/133363/files/texto_completo.pdf Versión publicada 2491382 http://zaguan.unizar.es/record/133363/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:133363 articulos driver 2026-02-17-20:22:07 ARTICLE