133374 20260217205504.0 doi 10.1016/j.jhazmat.2024.134030 sideral 138053 ART-2024-138053 eng Domenech, Josefa In vitro cell-transforming potential of secondary polyethylene terephthalate and polylactic acid nanoplastics 2024 Access copy available to the general public Unrestricted Continuous exposure to plastic pollutants may have serious consequences on human health. However, most toxicity assessments focus on non-environmentally relevant particles and rarely investigate long-term effects such as cancer induction. The present study assessed the carcinogenic potential of two secondary nanoplastics: polyethylene terephthalate (PET) particles generated from plastic bottles, and a biodegradable polylactic acid material, as respective examples of environmentally existing particles and new bioplastics. Pristine polystyrene nanoplastics were also included for comparison. A broad concentration range (6.25–200 μg/mL) of each nanoplastic was tested in both the initiation and promotion conditions of the regulatory assessment-accepted in vitro Bhas 42 cell transformation assay. Parallel cultures allowed confirmation of the efficient cellular internalisation of the three nanoplastics. Cell growth was enhanced by polystyrene in the initiation assay, and by PET in both conditions. Moreover, the number of transformed foci was significantly increased only by the highest PET concentration in the promotion assay, which also showed dose-dependency, indicating that nano PET can act as a non-genotoxic tumour promotor. Together, these findings support the carcinogenic risk assessment of nanoplastics and raise concerns regarding whether real-life co-exposure of PET nanoplastics and other environmental pollutants may result in synergistic transformation capacities. info:eu-repo/grantAgreement/EC/H2020/965196/EU/Innovative tools to study the impact and mode of action of micro and nanoplastics on human health: towards a knowledge base for risk assessment/PLASTICHEAL This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 965196-PLASTICHEAL info:eu-repo/grantAgreement/ES/MICINN/PID2020-116789-RB-C43 info:eu-repo/semantics/openAccess by-nc https://creativecommons.org/licenses/by-nc/4.0/deed.es 11.3 2024 ENVIRONMENTAL SCIENCES 19 / 376 = 0.051 2024 Q1 T1 ENGINEERING, ENVIRONMENTAL 5 / 83 = 0.06 2024 Q1 T1 3.078 2024 Environmental Chemistry 2024 Q1 Environmental Engineering 2024 Q1 Waste Management and Disposal 2024 Q1 Pollution 2024 Q1 Health, Toxicology and Mutagenesis 2024 Q1 24.6 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Villacorta, Aliro Ferrer, Juan Francisco Llorens-Chiralt, Raquel Marcos, Ricard Hernández, Alba Catalán, Julia Universidad de Zaragoza (orcid)0000-0003-2936-242X 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 469 (2024), 134030 [14 pp.] J. hazard. mater. Journal of Hazardous Materials 0304-3894 7710051 http://zaguan.unizar.es/record/133374/files/texto_completo.pdf Versión publicada 2008953 http://zaguan.unizar.es/record/133374/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:133374 articulos driver 2026-02-17-20:22:57 ARTICLE