133385 20260217205506.0 doi 10.1016/j.foodcont.2024.110482 sideral 138081 ART-2024-138081 eng Martínez, J.M. Universidad de Zaragoza (orcid)0000-0002-9337-417X Inactivation of Trichinella spp. in naturally infected boar meat after Pulsed Electric Field (PEF) treatments 2024 Access copy available to the general public Unrestricted Larvae of the nematode Trichinella are capable of causing parasitic infections in humans after the consumption of uncooked meat or meat products, leading to severe symptoms and even death. Meat inspection is costly and tends to progressively be derogated in holdings applying controlled housing conditions in certain regions. Our study focuses on the evaluation of the efficacy of Pulsed Electric Fields (PEF) in the inactivation of Trichinella spp., including excysted larvae isolated by artificial digestion and encapsulated larvae found in meat from naturally infected wild boars. Microscopic examination of unstained and propidium-iodide-stained larvae suspensions showed that most of the excysted larvae were inactivated 10 min after an intermediate PEF treatment (1 kV/cm, 0.41 kJ/kg). Treating excysted larvae with the mildest PEF treatment (0.5 kV/cm, 0.05 kJ/kg) combined with a 3% NaCl incubation resulted in synergistic inactivation. The application of 3 kV/cm (20 kJ/kg) to wild boar meat resulted in the inactivation of over 90% of encapsulated Trichinella larvae. The viability of Trichinella in meat was inversely correlated to the field strength applied (1–6 kV/cm) for equal energy input (20 kJ/kg). These findings demonstrate that PEF technology can potentially serve as a novel strategy for the inactivation of Trichinella larvae in meat. info:eu-repo/grantAgreement/ES/DGA/A03-20R info:eu-repo/grantAgreement/ES/DGA/LMP170_21 info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 6.3 2024 FOOD SCIENCE & TECHNOLOGY 24 / 182 = 0.132 2024 Q1 T1 1.265 2024 Food Science 2024 Q1 Biotechnology 2024 Q1 14.1 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Abad, V. Universidad de Zaragoza (orcid)0009-0000-8889-1263 Quílez, J. Universidad de Zaragoza (orcid)0000-0001-7831-2483 Reina, D. Pérez-Martin, J.E. Raso, J. Universidad de Zaragoza (orcid)0000-0003-3957-9091 Cebrián, G. Universidad de Zaragoza (orcid)0000-0002-5049-3646 Álvarez-Lanzarote, I. Universidad de Zaragoza (orcid)0000-0003-2430-858X 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 163 (2024), 110482 [7 pp.] Food control Food Control 0956-7135 5055011 http://zaguan.unizar.es/record/133385/files/texto_completo.pdf Versión publicada 2585401 http://zaguan.unizar.es/record/133385/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:133385 articulos driver 2026-02-17-20:23:26 ARTICLE