000133406 001__ 133406
000133406 005__ 20240720100902.0
000133406 0247_ $$2doi$$a10.1016/j.ejcb.2024.151396
000133406 0248_ $$2sideral$$a137830
000133406 037__ $$aART-2024-137830
000133406 041__ $$aeng
000133406 100__ $$0(orcid)0000-0001-7062-9099$$aGarcía-Gareta, Elena$$uUniversidad de Zaragoza
000133406 245__ $$aPhysico-chemical characterization of the tumour microenvironment of pancreatic ductal adenocarcinoma
000133406 260__ $$c2024
000133406 5060_ $$aAccess copy available to the general public$$fUnrestricted
000133406 5203_ $$aPancreatic ductal adenocarcinoma (PDAC) is a highly aggressive lethal malignancy that accounts for more than 90% of pancreatic cancer diagnoses. Our research is focused on the physico-chemical properties of the tumour microenvironment (TME), including its tumoural extracellular matrix (tECM), as they may have an important impact on the success of cancer therapies. PDAC xenografts and their decellularized tECM offer a great material source for research in terms of biomimicry with the original human tumour. Our aim was to evaluate and quantify the physico-chemical properties of the PDAC TME. Both cellularized (native TME) and decellularized (tECM) patient-derived PDAC xenografts were analyzed. A factorial design of experiments identified an optimal combination of factors for effective xenograft decellularization. Our results provide a complete advance in our understanding of the PDAC TME and its corresponding stroma, showing that it presents an interconnected porous architecture with very low permeability and small pores due to the contractility of the cellular components. This fact provides a potential therapeutic strategy based on the therapeutic agent size.
000133406 536__ $$9info:eu-repo/grantAgreement/ES/DGA/LMP29-21$$9info:eu-repo/grantAgreement/EC/H2020/101018587/EU/Individual and Collective Migration of the Immune Cellular System/ICoMICS$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 101018587-ICoMICS$$9info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI17-00082$$9info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI20-00921$$9info:eu-repo/grantAgreement/ES/MICINN/PID2020-113819RB-I00/AEI/10.13039/501100011033$$9info:eu-repo/grantAgreement/ES/MICINN/RYC2021-033490-I$$9info:eu-repo/grantAgreement/ES/MINECO-AEI-FEDER/PID2021-122409OB-C21$$9info:eu-repo/grantAgreement/ES/MS-ISCIII-FSE/CP16-00121
000133406 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000133406 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000133406 700__ $$aCalderón Villalba, Alejandro$$uUniversidad de Zaragoza
000133406 700__ $$0(orcid)0000-0003-1958-4432$$aAlamán-Díez, Pilar$$uUniversidad de Zaragoza
000133406 700__ $$aGracia Costa, Carlos
000133406 700__ $$0(orcid)0000-0003-2612-9235$$aGuerrero, Pedro Enrique$$uUniversidad de Zaragoza
000133406 700__ $$0(orcid)0009-0007-3057-6878$$aMur, Carlota$$uUniversidad de Zaragoza
000133406 700__ $$0(orcid)0009-0005-3783-9031$$aRueda Flores, Ana
000133406 700__ $$aOlivera Jurjo, Nerea
000133406 700__ $$aSancho, Patricia
000133406 700__ $$0(orcid)0000-0002-2901-4188$$aPérez, María Ángeles$$uUniversidad de Zaragoza
000133406 700__ $$0(orcid)0000-0002-9864-7683$$aGarcía-Aznar, José Manuel$$uUniversidad de Zaragoza
000133406 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000133406 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000133406 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000133406 7102_ $$15001$$2065$$aUniversidad de Zaragoza$$bDpto. Ciencia Tecnol.Mater.Fl.$$cÁrea Cienc.Mater. Ingen.Metal.
000133406 773__ $$g103, 2 (2024), 151396 [16 pp.]$$pEur. j. cell biol.$$tEuropean Journal of Cell Biology$$x0171-9335
000133406 8564_ $$s1572072$$uhttps://zaguan.unizar.es/record/133406/files/texto_completo.pdf$$yVersión publicada
000133406 8564_ $$s2667074$$uhttps://zaguan.unizar.es/record/133406/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000133406 909CO $$ooai:zaguan.unizar.es:133406$$particulos$$pdriver
000133406 951__ $$a2024-07-19-18:53:06
000133406 980__ $$aARTICLE