133406 20260217205514.0 doi 10.1016/j.ejcb.2024.151396 sideral 137830 ART-2024-137830 eng García-Gareta, Elena Universidad de Zaragoza (orcid)0000-0001-7062-9099 Physico-chemical characterization of the tumour microenvironment of pancreatic ductal adenocarcinoma 2024 Access copy available to the general public Unrestricted Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive lethal malignancy that accounts for more than 90% of pancreatic cancer diagnoses. Our research is focused on the physico-chemical properties of the tumour microenvironment (TME), including its tumoural extracellular matrix (tECM), as they may have an important impact on the success of cancer therapies. PDAC xenografts and their decellularized tECM offer a great material source for research in terms of biomimicry with the original human tumour. Our aim was to evaluate and quantify the physico-chemical properties of the PDAC TME. Both cellularized (native TME) and decellularized (tECM) patient-derived PDAC xenografts were analyzed. A factorial design of experiments identified an optimal combination of factors for effective xenograft decellularization. Our results provide a complete advance in our understanding of the PDAC TME and its corresponding stroma, showing that it presents an interconnected porous architecture with very low permeability and small pores due to the contractility of the cellular components. This fact provides a potential therapeutic strategy based on the therapeutic agent size. info:eu-repo/grantAgreement/ES/DGA/LMP29-21 info:eu-repo/grantAgreement/EC/H2020/101018587/EU/Individual and Collective Migration of the Immune Cellular System/ICoMICS This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 101018587-ICoMICS info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI17-00082 info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI20-00921 info:eu-repo/grantAgreement/ES/MICINN/PID2020-113819RB-I00/AEI/10.13039/501100011033 info:eu-repo/grantAgreement/ES/MICINN/RYC2021-033490-I info:eu-repo/grantAgreement/ES/MINECO-AEI-FEDER/PID2021-122409OB-C21 info:eu-repo/grantAgreement/ES/MS-ISCIII-FSE/CP16-00121 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 4.3 2024 CELL BIOLOGY 81 / 204 = 0.397 2024 Q2 T2 1.491 2024 Histology 2024 Q1 Pathology and Forensic Medicine 2024 Q1 Medicine (miscellaneous) 2024 Q1 Cell Biology 2024 Q2 6.3 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Calderón Villalba, Alejandro Universidad de Zaragoza Alamán-Díez, Pilar Universidad de Zaragoza (orcid)0000-0003-1958-4432 Gracia Costa, Carlos Guerrero, Pedro Enrique Universidad de Zaragoza (orcid)0000-0003-2612-9235 Mur, Carlota Universidad de Zaragoza (orcid)0009-0007-3057-6878 Rueda Flores, Ana Olivera Jurjo, Nerea Sancho, Patricia Pérez, María Ángeles Universidad de Zaragoza (orcid)0000-0002-2901-4188 García-Aznar, José Manuel Universidad de Zaragoza (orcid)0000-0002-9864-7683 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 5001 065 Universidad de Zaragoza Dpto. Ciencia Tecnol.Mater.Fl. Área Cienc.Mater. Ingen.Metal. 103, 2 (2024), 151396 [16 pp.] Eur. j. cell biol. European Journal of Cell Biology 0171-9335 1572072 http://zaguan.unizar.es/record/133406/files/texto_completo.pdf Versión publicada 2667074 http://zaguan.unizar.es/record/133406/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:133406 articulos driver 2026-02-17-20:26:10 ARTICLE