133442 20260217205500.0 doi 10.1016/j.mrgentox.2024.503753 sideral 138120 ART-2024-138120 eng Catalán, Julia Universidad de Zaragoza (orcid)0000-0003-2936-242X Chromosome-specific induction of micronuclei and chromosomal aberrations by mitomycin C: Involvement of human chromosomes 9, 1 and 16 2024 Access copy available to the general public Unrestricted Cytogenetic studies have shown that human chromosomes 1, 9, and 16, with a large heterochromatic region of highly methylated classical satellite DNA, are prone to induction of chromatid breaks and interchanges by mitomycin C (MMC). A couple of studies have indicated that material from chromosome 9, and possibly also from chromosomes 1 and 16, are preferentially micronucleated by MMC. Here, we further examined the chromosome-specific induction of micronuclei (MN; with and without cytochalasin B) and chromosomal aberrations (CAs) by MMC. Cultures of isolated human lymphocytes from two male donors were treated (at 48 h of culture, for 24 h) with MMC (500 ng/ml), and the induced MN were examined by a pancentromeric DNA probe and paint probe for chromosome 9, and by paint probes for chromosomes 1 and 16. MMC increased the total frequency of MN by 6–8-fold but the frequency of chromosome 9 -positive (9+) MN by 29–30-fold and the frequency of chromosome 1 -positive (1+) MN and chromosome 16 -positive (16+) MN by 12–16-fold and 10–17-fold, respectively. After treatment with MMC, 34–47 % of all MN were 9+, 17–20 % 1+, and 3–4 % 16+. The majority (94–96 %) of the 9+ MN contained no centromere and thus harboured acentric fragments. When MMC-induced CAs aberrations were characterized by using the pancentromeric DNA probe and probes for the classical satellite region and long- and short- arm telomeres of chromosome 9, a high proportion of chromosomal breaks (31 %) and interchanges (41 %) concerned chromosome 9. In 83 % of cases, the breakpoint in chromosome 9 was just below the region (9cen-q12) labelled by the classical satellite probe. Our results indicate that MMC specifically induces MN harbouring fragments of chromosome 9, 1, and 16. CAs of chromosome 9 are highly overrepresented in metaphases of MMC-treated lymphocytes. The preferential breakpoint is below the region 9q12. info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 2.5 2024 GENETICS & HEREDITY 95 / 192 = 0.495 2024 Q2 T2 TOXICOLOGY 66 / 106 = 0.623 2024 Q3 T2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY 104 / 177 = 0.588 2024 Q3 T2 0.668 2024 Health, Toxicology and Mutagenesis 2024 Q2 Genetics 2024 Q3 4.4 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Järventaus, Hilkka Falck, Ghita C.-M. Moreno, Carlos Universidad de Zaragoza (orcid)0000-0002-1946-1187 Norppa, Hannu 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 896 (2024), 503753 [9 pp.] Mutat. res., Genet. toxicol. environ. mutagen. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 1383-5718 1694934 http://zaguan.unizar.es/record/133442/files/texto_completo.pdf Versión publicada 2476480 http://zaguan.unizar.es/record/133442/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:133442 articulos driver 2026-02-17-20:21:49 ARTICLE