000133450 001__ 133450
000133450 005__ 20240416133904.0
000133450 0247_ $$2doi$$a10.3390/ijms25074020
000133450 0248_ $$2sideral$$a138138
000133450 037__ $$aART-2024-138138
000133450 041__ $$aeng
000133450 100__ $$aFernandez-Carro, Estibaliz$$uUniversidad de Zaragoza
000133450 245__ $$aHuman Dermal Decellularized ECM Hydrogels as Scaffolds for 3D In Vitro Skin Aging Models
000133450 260__ $$c2024
000133450 5060_ $$aAccess copy available to the general public$$fUnrestricted
000133450 5203_ $$aBiomaterials play an important role in the development of advancing three dimensional (3D) in vitro skin models, providing valuable insights for drug testing and tissue-specific modeling. Commercial materials, such as collagen, fibrin or alginate, have been widely used in skin modeling. However, they do not adequately represent the molecular complexity of skin components. On this regard, the development of novel biomaterials that represent the complexity of tissues is becoming more important in the design of advanced models. In this study, we have obtained aged human decellularized dermal extracellular matrix (dECM) hydrogels extracted from cadaveric human skin and demonstrated their potential as scaffold for advanced skin models. These dECM hydrogels effectively reproduce the complex fibrillar structure of other common scaffolds, exhibiting similar mechanical properties, while preserving the molecular composition of the native dermis. It is worth noting that fibroblasts embedded within human dECM hydrogels exhibit a behavior more representative of natural skin compared to commercial collagen hydrogels, where uncontrolled cell proliferation leads to material shrinkage. The described human dECM hydrogel is able to be used as scaffold for dermal fibroblasts in a skin aging-on-a-chip model. These results demonstrate that dECM hydrogels preserve essential components of the native human dermis making them a suitable option for the development of 3D skin aging models that accurately represent the cellular microenvironment, improving existing in vitro skin models and allowing for more reliable results in dermatopathological studies.
000133450 536__ $$9info:eu-repo/grantAgreement/ES/DGA/LMP233_21$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2017-84087-R$$9info:eu-repo/grantAgreement/ES/MINECO-FSE-FEDER/RYC-2015-18471$$9info:eu-repo/grantAgreement/ES/MINECO/PID2020-113003GB-I00
000133450 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000133450 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000133450 700__ $$0(orcid)0000-0002-1075-8267$$aRemacha, Ana Rosa
000133450 700__ $$aOrera, Irene
000133450 700__ $$aLattanzio, Giuseppe
000133450 700__ $$0(orcid)0000-0001-5560-3771$$aGarcia-Barrios, Alberto$$uUniversidad de Zaragoza
000133450 700__ $$0(orcid)0000-0002-5380-6863$$adel Barrio, Jesús$$uUniversidad de Zaragoza
000133450 700__ $$0(orcid)0000-0002-7854-8856$$aAlcaine, Clara$$uUniversidad de Zaragoza
000133450 700__ $$0(orcid)0000-0002-8666-622X$$aCiriza, Jesús$$uUniversidad de Zaragoza
000133450 7102_ $$11006$$2255$$aUniversidad de Zaragoza$$bDpto. Fisiatría y Enfermería$$cÁrea Enfermería
000133450 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000133450 7102_ $$11003$$2027$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Anatom.Embriol.Humana
000133450 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000133450 773__ $$g25, 7 (2024), 4020 [15 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000133450 8564_ $$s2586320$$uhttps://zaguan.unizar.es/record/133450/files/texto_completo.pdf$$yVersión publicada
000133450 8564_ $$s2626685$$uhttps://zaguan.unizar.es/record/133450/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000133450 909CO $$ooai:zaguan.unizar.es:133450$$particulos$$pdriver
000133450 951__ $$a2024-04-16-13:15:41
000133450 980__ $$aARTICLE