133450 20260217205501.0 doi 10.3390/ijms25074020 sideral 138138 ART-2024-138138 eng Fernandez-Carro, Estibaliz Universidad de Zaragoza Human Dermal Decellularized ECM Hydrogels as Scaffolds for 3D In Vitro Skin Aging Models 2024 Access copy available to the general public Unrestricted Biomaterials play an important role in the development of advancing three dimensional (3D) in vitro skin models, providing valuable insights for drug testing and tissue-specific modeling. Commercial materials, such as collagen, fibrin or alginate, have been widely used in skin modeling. However, they do not adequately represent the molecular complexity of skin components. On this regard, the development of novel biomaterials that represent the complexity of tissues is becoming more important in the design of advanced models. In this study, we have obtained aged human decellularized dermal extracellular matrix (dECM) hydrogels extracted from cadaveric human skin and demonstrated their potential as scaffold for advanced skin models. These dECM hydrogels effectively reproduce the complex fibrillar structure of other common scaffolds, exhibiting similar mechanical properties, while preserving the molecular composition of the native dermis. It is worth noting that fibroblasts embedded within human dECM hydrogels exhibit a behavior more representative of natural skin compared to commercial collagen hydrogels, where uncontrolled cell proliferation leads to material shrinkage. The described human dECM hydrogel is able to be used as scaffold for dermal fibroblasts in a skin aging-on-a-chip model. These results demonstrate that dECM hydrogels preserve essential components of the native human dermis making them a suitable option for the development of 3D skin aging models that accurately represent the cellular microenvironment, improving existing in vitro skin models and allowing for more reliable results in dermatopathological studies. info:eu-repo/grantAgreement/ES/DGA/LMP233_21 info:eu-repo/grantAgreement/ES/MINECO/CTQ2017-84087-R info:eu-repo/grantAgreement/ES/MINECO-FSE-FEDER/RYC-2015-18471 info:eu-repo/grantAgreement/ES/MINECO/PID2020-113003GB-I00 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 4.9 2024 BIOCHEMISTRY & MOLECULAR BIOLOGY 72 / 320 = 0.225 2024 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 70 / 239 = 0.293 2024 Q2 T1 1.273 2024 Medicine (miscellaneous) 2024 Q1 Physical and Theoretical Chemistry 2024 Q1 Computer Science Applications 2024 Q1 Inorganic Chemistry 2024 Q1 Spectroscopy 2024 Q1 Organic Chemistry 2024 Q1 Molecular Biology 2024 Q2 Catalysis 2024 Q2 9.0 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Remacha, Ana Rosa (orcid)0000-0002-1075-8267 Orera, Irene Lattanzio, Giuseppe Garcia-Barrios, Alberto Universidad de Zaragoza (orcid)0000-0001-5560-3771 del Barrio, Jesús Universidad de Zaragoza (orcid)0000-0002-5380-6863 Alcaine, Clara Universidad de Zaragoza (orcid)0000-0002-7854-8856 Ciriza, Jesús Universidad de Zaragoza (orcid)0000-0002-8666-622X 1006 255 Universidad de Zaragoza Dpto. Fisiatría y Enfermería Área Enfermería 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 1003 027 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Anatom.Embriol.Humana 2013 765 Universidad de Zaragoza Dpto. Química Orgánica Área Química Orgánica 25, 7 (2024), 4020 [15 pp.] Int. j. mol. sci. International Journal of Molecular Sciences 1661-6596 2586320 http://zaguan.unizar.es/record/133450/files/texto_completo.pdf Versión publicada 2626685 http://zaguan.unizar.es/record/133450/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:133450 articulos driver 2026-02-17-20:21:55 ARTICLE