000134479 001__ 134479
000134479 005__ 20240424142048.0
000134479 037__ $$aTAZ-TFG-2023-3645
000134479 041__ $$aeng
000134479 1001_ $$aFerrer Royo, Silvia
000134479 24200 $$aSetting up an in vitro system to analyze the effect of fluid pressure on the growth and morphology of pancreatic tumor cells grown in 3D.
000134479 24500 $$aPuesta a punto de un modelo in vitro para analizar el efecto de la presión en el crecimiento y morfología de células pancreáticas tumorales crecidas en 3D.
000134479 260__ $$aZaragoza$$bUniversidad de Zaragoza$$c2023
000134479 506__ $$aby-nc-sa$$bCreative Commons$$c3.0$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/
000134479 520__ $$aUp until today, pancreatic cancer is one of the deadliest and fastest-killing cancers. A late diagnosis, the lack of effective therapies and the tumor’s chemoresistance are the principal causes of such elevated mortality rates. Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer and its noticeable resistance to chemotherapy drugs is favored by its dense stroma and high interstitial fluid pressure (IFP). In this work, it was our objective to design a system that allowed us to recreate in vitro the IFP to which PDAC cells are subjected and to evaluate the effect IFP has on their growth and morphology. For this purpose, we created a 3D printed system with which we were able to exert a maximum IFP of 29.3 mmHg over microfluidic 3D cultures of two PDAC cell lines (PANC-1 and PDX 354-gfp). Spheroids size, morphology, growth rate and distribution according to their area were analyzed for 3D cultures under different fluid pressure conditions. The results obtained suggested that PANC-1 cells grow better with an IFP of 29.3 mmHg but were inconclusive in regard to the PDX cell line used. Additionally, we tested the possibility of drug supply to tumorous cells grown in microchips using our 3D printed system with positive results.<br /><br />
000134479 521__ $$aGraduado en Biotecnología
000134479 540__ $$aDerechos regulados por licencia Creative Commons
000134479 700__ $$aGonzález Loyola, Alejandra$$edir.
000134479 700__ $$aGarcía Aznar, José Manuel$$edir.
000134479 7102_ $$aUniversidad de Zaragoza$$bIngeniería Mecánica$$cIngeniería Mecánica
000134479 8560_ $$f799856@unizar.es
000134479 8564_ $$s46358080$$uhttps://zaguan.unizar.es/record/134479/files/TAZ-TFG-2023-3645.pdf$$yMemoria (eng)
000134479 8564_ $$s52437$$uhttps://zaguan.unizar.es/record/134479/files/TAZ-TFG-2023-3645_ANE.pdf$$yAnexos (eng)
000134479 909CO $$ooai:zaguan.unizar.es:134479$$pdriver$$ptrabajos-fin-grado
000134479 950__ $$a
000134479 951__ $$adeposita:2024-04-24
000134479 980__ $$aTAZ$$bTFG$$cCIEN
000134479 999__ $$a20230901113122.CREATION_DATE