000134596 001__ 134596
000134596 005__ 20240424142035.0
000134596 0247_ $$2doi$$a10.1016/j.therwi.2024.100075
000134596 0248_ $$2sideral$$a138279
000134596 037__ $$aART-2024-138279
000134596 041__ $$aeng
000134596 100__ $$aMartínez de los Reyes, Nuria
000134596 245__ $$aOptimized heterologous in vitro fertilization with Iberian ibex sperm and domestic goat oocytes
000134596 260__ $$c2024
000134596 5060_ $$aAccess copy available to the general public$$fUnrestricted
000134596 5203_ $$aAssisted reproductive technologies are key to maintain genetic stocks of endangered wild species, such as the Iberian ibex (Capra pyrenaica). Due to the low availability of ibex ovaries, heterologous in vitro fertilization (IVF) with domestic goat (Capra hircus) oocytes constitutes an excellent alternative to determine the fertilization capacity of ibex sperm doses. The aim of this study was to optimize heterologous ibex-goat IVF procedures by
testing two different IVF media (TALP and SOF) and to determine whether estrous sheep serum (ESS) is required for fertilization. We found that TALP medium provides optimal conditions to conduct heterologous ibex-goat IVF, yielding blastocyst rates above 50%, and that supplementation with ESS is not required. No differences were found in embryo quality between embryos fertilized in TALP, SOF alone, or SOF supplemented with 2 or 20% ESS, based on the analysis of cell lineages development at day (D) 8 and D10 of development. Optimized heterologous ibex-goat IVF, together with embryo quality assessment through lineages development analysis, constitute an excellent system to assess fertilization capacity of ibex sperm doses, and opens the possibility of
performing homologous in vitro embryo production in this species.
000134596 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-122153NA-I00
000134596 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000134596 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000134596 700__ $$0(orcid)0000-0003-3929-5064$$aCarvajal-Serna, Melissa$$uUniversidad de Zaragoza
000134596 700__ $$aFlores-Borobia, Inés
000134596 700__ $$aMarigorta, Pilar
000134596 700__ $$aPeris-Frau, Patricia
000134596 700__ $$aSantiago-Moreno, Julián
000134596 700__ $$aBermejo-Álvarez, Pablo
000134596 700__ $$aRamos-Ibeas, Priscila
000134596 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología
000134596 773__ $$g4 (2024), 100075$$tTheriogenology Wild$$x2773-093X
000134596 8564_ $$s7814935$$uhttps://zaguan.unizar.es/record/134596/files/texto_completo.pdf$$yVersión publicada
000134596 8564_ $$s2436541$$uhttps://zaguan.unizar.es/record/134596/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000134596 909CO $$ooai:zaguan.unizar.es:134596$$particulos$$pdriver
000134596 951__ $$a2024-04-24-13:24:17
000134596 980__ $$aARTICLE