000134887 001__ 134887 000134887 005__ 20240503133220.0 000134887 0247_ $$2doi$$a10.1080/01652176.2024.2318195 000134887 0248_ $$2sideral$$a138328 000134887 037__ $$aART-2024-138328 000134887 041__ $$aeng 000134887 100__ $$0(orcid)0000-0001-6209-4282$$aVillanueva-Saz, Sergio$$uUniversidad de Zaragoza 000134887 245__ $$aSerum protein electrophoresis in European mink (Mustela lutreola): reference intervals and comparison of agarose gel electrophoresis and capillary zone electrophoresis 000134887 260__ $$c2024 000134887 5060_ $$aAccess copy available to the general public$$fUnrestricted 000134887 5203_ $$aBackground Knowledge of reference intervals for blood analytes, including serum protein fractions, is of great importance for the identification of infectious and inflammatory diseases and is often lacking in wild animal species. Material and methods Serum samples were obtained from European minks enrolled in the breeding program (n = 55). Agarose gel electrophoresis (AGE) and capillary zone electrophoresis (CZE) were used to separate and identify protein fractions. Albumin, α1, α2, β, and γ-globulins fractions were identified in all mink sera by both electrophoresis methods. Reference intervals (90% CI) were determined following the 2008 guidelines of the Clinical Laboratory Standard Institute. The methods were compared using Passing-Bablok regression, Bland–Altman analysis, and Lin’s concordance correlation. Results A significant bias was found between methods for α1, α2, and γ-globulin. Lin’s concordance correlation was considered unacceptable for α1, α2, and β-globulins. Differences for gender between methods were found for albumin and α2-globuins, which were higher for males than females. γ-globulins were higher for adults than young minks using both methods; however, α1 and α2-globulins were lower. Conclusion Both methods are adequate for identifying serum protein disorders, but the AGE and CZE methods are not equivalent. Therefore, reference intervals for each technique are required. 000134887 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/ 000134887 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000134887 700__ $$aAranda, María del Carmen 000134887 700__ $$aJiménez, María de los Ángeles 000134887 700__ $$ade Andrés, Paloma Jimena 000134887 700__ $$0(orcid)0000-0003-2957-1379$$aVerde, Maite$$uUniversidad de Zaragoza 000134887 700__ $$0(orcid)0000-0002-0606-0763$$aCliment, María$$uUniversidad de Zaragoza 000134887 700__ $$0(orcid)0000-0001-5816-6167$$aLebrero Berna, María Eugenia$$uUniversidad de Zaragoza 000134887 700__ $$aMarteles Aragüés, Diana$$uUniversidad de Zaragoza 000134887 700__ $$0(orcid)0000-0002-2557-4890$$aFernández, Antonio$$uUniversidad de Zaragoza 000134887 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com 000134887 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal 000134887 773__ $$g44, 1 (2024), [11 pp.]$$pVet. q.$$tVETERINARY QUARTERLY$$x0165-2176 000134887 8564_ $$s1548997$$uhttps://zaguan.unizar.es/record/134887/files/texto_completo.pdf$$yVersión publicada 000134887 8564_ $$s2811565$$uhttps://zaguan.unizar.es/record/134887/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000134887 909CO $$ooai:zaguan.unizar.es:134887$$particulos$$pdriver 000134887 951__ $$a2024-05-03-11:07:01 000134887 980__ $$aARTICLE