134887 20260217205516.0 doi 10.1080/01652176.2024.2318195 sideral 138328 ART-2024-138328 eng Villanueva-Saz, Sergio Universidad de Zaragoza (orcid)0000-0001-6209-4282 Serum protein electrophoresis in European mink (Mustela lutreola): reference intervals and comparison of agarose gel electrophoresis and capillary zone electrophoresis 2024 Access copy available to the general public Unrestricted Background Knowledge of reference intervals for blood analytes, including serum protein fractions, is of great importance for the identification of infectious and inflammatory diseases and is often lacking in wild animal species. Material and methods Serum samples were obtained from European minks enrolled in the breeding program (n = 55). Agarose gel electrophoresis (AGE) and capillary zone electrophoresis (CZE) were used to separate and identify protein fractions. Albumin, α1, α2, β, and γ-globulins fractions were identified in all mink sera by both electrophoresis methods. Reference intervals (90% CI) were determined following the 2008 guidelines of the Clinical Laboratory Standard Institute. The methods were compared using Passing-Bablok regression, Bland–Altman analysis, and Lin’s concordance correlation. Results A significant bias was found between methods for α1, α2, and γ-globulin. Lin’s concordance correlation was considered unacceptable for α1, α2, and β-globulins. Differences for gender between methods were found for albumin and α2-globuins, which were higher for males than females. γ-globulins were higher for adults than young minks using both methods; however, α1 and α2-globulins were lower. Conclusion Both methods are adequate for identifying serum protein disorders, but the AGE and CZE methods are not equivalent. Therefore, reference intervals for each technique are required. info:eu-repo/semantics/openAccess by-nc https://creativecommons.org/licenses/by-nc/4.0/deed.es 5.2 2024 VETERINARY SCIENCES 5 / 170 = 0.029 2024 Q1 T1 1.308 2024 Veterinary (miscellaneous) 2024 Q1 Medicine (miscellaneous) 2024 Q1 7.8 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Aranda, María del Carmen Jiménez, María de los Ángeles de Andrés, Paloma Jimena Verde, Maite Universidad de Zaragoza (orcid)0000-0003-2957-1379 Climent, María Universidad de Zaragoza (orcid)0000-0002-0606-0763 Lebrero Berna, María Eugenia Universidad de Zaragoza (orcid)0000-0001-5816-6167 Marteles Aragüés, Diana Universidad de Zaragoza Fernández, Antonio Universidad de Zaragoza (orcid)0000-0002-2557-4890 1001 025 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Anatom.Anatom.Patológ.Com 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 44, 1 (2024), [11 pp.] Vet. q. VETERINARY QUARTERLY 0165-2176 1548997 http://zaguan.unizar.es/record/134887/files/texto_completo.pdf Versión publicada 2811565 http://zaguan.unizar.es/record/134887/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:134887 articulos driver 2026-02-17-20:26:54 ARTICLE