000134957 001__ 134957
000134957 005__ 20240509150148.0
000134957 0247_ $$2doi$$a10.3390/biomimetics9050262
000134957 0248_ $$2sideral$$a138496
000134957 037__ $$aART-2024-138496
000134957 041__ $$aeng
000134957 100__ $$aOlaizola-Rodrigo, Claudia
000134957 245__ $$aReducing Inert Materials for Optimal Cell–Cell and Cell–Matrix Interactions within Microphysiological Systems
000134957 260__ $$c2024
000134957 5060_ $$aAccess copy available to the general public$$fUnrestricted
000134957 5203_ $$aIn the pursuit of achieving a more realistic in vitro simulation of human biological tissues, microfluidics has emerged as a promising technology. Organ-on-a-chip (OoC) devices, a product of this technology, contain miniature tissues within microfluidic chips, aiming to closely mimic the in vivo environment. However, a notable drawback is the presence of inert material between compartments, hindering complete contact between biological tissues. Current membranes, often made of PDMS or plastic materials, prevent full interaction between cell types and nutrients. Furthermore, their non-physiological mechanical properties and composition may induce unexpected cell responses. Therefore, it is essential to minimize the contact area between cells and the inert materials while simultaneously maximizing the direct contact between cells and matrices in different compartments. The main objective of this work is to minimize inert materials within the microfluidic chip while preserving proper cellular distribution. Two microfluidic devices were designed, each with a specific focus on maximizing direct cell–matrix or cell–cell interactions. The first chip, designed to increase direct cell–cell interactions, incorporates a nylon mesh with regular pores of 150 microns. The second chip minimizes interference from inert materials, thereby aiming to increase direct cell–matrix contact. It features an inert membrane with optimized macropores of 1 mm of diameter for collagen hydrogel deposition. Biological validation of both devices has been conducted through the implementation of cell migration and cell-to-cell interaction assays, as well as the development of epithelia, from isolated cells or spheroids. This endeavor contributes to the advancement of microfluidic technology, aimed at enhancing the precision and biological relevance of in vitro simulations in pursuit of more biomimetic models.
000134957 536__ $$9info:eu-repo/grantAgreement/ES/AEI/PID2021-126051OB-C41$$9info:eu-repo/grantAgreement/ES/DGA/T62-230R$$9info:eu-repo/grantAgreement/EC/H2020/778354/EU/Heart On chip based on induced pluripotent Stem cell Technology for personalized Medicine/CISTEM$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 778354-CISTEM$$9info:eu-repo/grantAgreement/EC/H2020/876190/EU/Accelerating Innovation in Microfabricated Medical Devices/Moore4Medical$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 876190-Moore4Medical$$9info:eu-repo/grantAgreement/ES/MINECO/DIN2020-011544
000134957 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000134957 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000134957 700__ $$aCastro-Abril, Héctor
000134957 700__ $$aPerisé-Badía, Ismael
000134957 700__ $$aPancorbo, Lara
000134957 700__ $$0(orcid)0000-0003-2410-5678$$aOchoa, Ignacio$$uUniversidad de Zaragoza
000134957 700__ $$aMonge, Rosa
000134957 700__ $$0(orcid)0000-0003-0156-4230$$aOliván, Sara$$uUniversidad de Zaragoza
000134957 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000134957 773__ $$g9, 5 (2024), 262 [19 pp.]$$tBiomimetics$$x2313-7673
000134957 8564_ $$s13921084$$uhttps://zaguan.unizar.es/record/134957/files/texto_completo.pdf$$yVersión publicada
000134957 8564_ $$s2618919$$uhttps://zaguan.unizar.es/record/134957/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000134957 909CO $$ooai:zaguan.unizar.es:134957$$particulos$$pdriver
000134957 951__ $$a2024-05-09-13:05:47
000134957 980__ $$aARTICLE