000135157 001__ 135157 000135157 005__ 20240516095249.0 000135157 0247_ $$2doi$$a10.3390/cells13080691 000135157 0248_ $$2sideral$$a138549 000135157 037__ $$aART-2024-138549 000135157 041__ $$aeng 000135157 100__ $$0(orcid)0000-0001-8762-5457$$aSancho-Albero, Maria$$uUniversidad de Zaragoza 000135157 245__ $$aExtracellular Vesicles-Mediated Bio-Orthogonal Catalysis in Growing Tumors 000135157 260__ $$c2024 000135157 5060_ $$aAccess copy available to the general public$$fUnrestricted 000135157 5203_ $$aSeveral studies have reported the successful use of bio-orthogonal catalyst nanoparticles (NPs) for cancer therapy. However, the delivery of the catalysts to the target tissues in vivo remains an unsolved challenge. The combination of catalytic NPs with extracellular vesicles (EVs) has been proposed as a promising approach to improve the delivery of therapeutic nanomaterials to the desired organs. In this study, we have developed a nanoscale bio-hybrid vector using a CO-mediated reduction at low temperature to generate ultrathin catalytic Pd nanosheets (PdNSs) as catalysts directly inside cancer-derived EVs. We have also compared their biodistribution with that of PEGylated PdNSs delivered by the EPR effect. Our results indicate that the accumulation of PdNSs in the tumour tissue was significantly higher when they were administered within the EVs compared to the PEGylated PdNSs. Conversely, the amount of Pd found in non-target organs (i.e., liver) was lowered. Once the Pd-based catalytic EVs were accumulated in the tumours, they enabled the activation of a paclitaxel prodrug demonstrating their ability to carry out bio-orthogonal uncaging chemistries in vivo for cancer therapy. 000135157 536__ $$9info:eu-repo/grantAgreement/EC/H2020/742684/EU/Catalytic Dual-Function Devices Against Cancer/CADENCE$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 742684-CADENCE 000135157 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000135157 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000135157 700__ $$0(orcid)0000-0002-6873-5244$$aSebastian, Victor$$uUniversidad de Zaragoza 000135157 700__ $$aPerez-Lopez, Ana M. 000135157 700__ $$0(orcid)0000-0003-4160-9720$$aMartin-Duque, Pilar$$uUniversidad de Zaragoza 000135157 700__ $$aUnciti-Broceta, Asier 000135157 700__ $$0(orcid)0000-0002-8701-9745$$aSantamaria, Jesus$$uUniversidad de Zaragoza 000135157 7102_ $$11013$$2275$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Estomatología 000135157 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química 000135157 773__ $$g13, 8 (2024), 691 [15 pp.]$$pCells$$tCells$$x2073-4409 000135157 8564_ $$s2670948$$uhttps://zaguan.unizar.es/record/135157/files/texto_completo.pdf$$yVersión publicada 000135157 8564_ $$s2617999$$uhttps://zaguan.unizar.es/record/135157/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000135157 909CO $$ooai:zaguan.unizar.es:135157$$particulos$$pdriver 000135157 951__ $$a2024-05-16-08:53:13 000135157 980__ $$aARTICLE