135157 20260217205535.0 doi 10.3390/cells13080691 sideral 138549 ART-2024-138549 eng Sancho-Albero, Maria Universidad de Zaragoza (orcid)0000-0001-8762-5457 Extracellular Vesicles-Mediated Bio-Orthogonal Catalysis in Growing Tumors 2024 Access copy available to the general public Unrestricted Several studies have reported the successful use of bio-orthogonal catalyst nanoparticles (NPs) for cancer therapy. However, the delivery of the catalysts to the target tissues in vivo remains an unsolved challenge. The combination of catalytic NPs with extracellular vesicles (EVs) has been proposed as a promising approach to improve the delivery of therapeutic nanomaterials to the desired organs. In this study, we have developed a nanoscale bio-hybrid vector using a CO-mediated reduction at low temperature to generate ultrathin catalytic Pd nanosheets (PdNSs) as catalysts directly inside cancer-derived EVs. We have also compared their biodistribution with that of PEGylated PdNSs delivered by the EPR effect. Our results indicate that the accumulation of PdNSs in the tumour tissue was significantly higher when they were administered within the EVs compared to the PEGylated PdNSs. Conversely, the amount of Pd found in non-target organs (i.e., liver) was lowered. Once the Pd-based catalytic EVs were accumulated in the tumours, they enabled the activation of a paclitaxel prodrug demonstrating their ability to carry out bio-orthogonal uncaging chemistries in vivo for cancer therapy. info:eu-repo/grantAgreement/EC/H2020/742684/EU/Catalytic Dual-Function Devices Against Cancer/CADENCE This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 742684-CADENCE info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 5.2 2024 CELL BIOLOGY 59 / 204 = 0.289 2024 Q2 T1 1.67 2024 Biochemistry, Genetics and Molecular Biology (miscellaneous) 2024 Q1 10.5 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Sebastian, Victor Universidad de Zaragoza (orcid)0000-0002-6873-5244 Perez-Lopez, Ana M. Martin-Duque, Pilar Universidad de Zaragoza (orcid)0000-0003-4160-9720 Unciti-Broceta, Asier Santamaria, Jesus Universidad de Zaragoza (orcid)0000-0002-8701-9745 1013 275 Universidad de Zaragoza Dpto. Cirugía Área Estomatología 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 13, 8 (2024), 691 [15 pp.] Cells Cells 2073-4409 2670948 http://zaguan.unizar.es/record/135157/files/texto_completo.pdf Versión publicada 2617999 http://zaguan.unizar.es/record/135157/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:135157 articulos driver 2026-02-17-20:33:56 ARTICLE