000135487 001__ 135487 000135487 005__ 20260112133306.0 000135487 0247_ $$2doi$$a10.3389/fpls.2024.1386023 000135487 0248_ $$2sideral$$a138642 000135487 037__ $$aART-2024-138642 000135487 041__ $$aeng 000135487 100__ $$aClaver, Ana 000135487 245__ $$aTranscriptomic and lipidomic analysis of the differential pathway contribution to the incorporation of erucic acid to triacylglycerol during Pennycress seed maturation 000135487 260__ $$c2024 000135487 5060_ $$aAccess copy available to the general public$$fUnrestricted 000135487 5203_ $$aThlaspi arvense (Pennycress) is an emerging feedstock for biofuel production because of its high seed oil content enriched in erucic acid. A transcriptomic and a lipidomic study were performed to analyze the dynamics of gene expression, glycerolipid content and acyl-group distribution during seed maturation. Genes involved in fatty acid biosynthesis were expressed at the early stages of seed maturation. Genes encoding enzymes of the Kennedy pathway like diacylglycerol acyltransferase1 (TaDGAT1), lysophosphatidic acid acyltransferase (TaLPAT) or glycerol 3-phosphate acyltransferase (TaGPAT) increased their expression with maturation, coinciding with the increase in triacylglycerol species containing 22:1. Positional analysis showed that the most abundant triacylglycerol species contained 18:2 at sn-2 position in all maturation stages, suggesting no specificity of the lysophosphatidic acid acyltransferase for very long chain fatty acids. Diacylglycerol acyltransferase2 (TaDGAT2) mRNA was more abundant at the initial maturation stages, coincident with the rapid incorporation of 22:1 to triacylglycerol, suggesting a coordination between Diacylglycerol acyltransferase enzymes for triacylglycerol biosynthesis. Genes encoding the phospholipid-diacylglycerol acyltransferase (TaPDAT1), lysophosphatidylcholine acyltransferase (TaLPCAT) or phosphatidylcholine diacylglycerolcholine phosphotransferase (TaPDCT), involved in acyl-editing or phosphatidyl-choline (PC)-derived diacylglycerol (DAG) biosynthesis showed also higher expression at the early maturation stages, coinciding with a higher proportion of triacylglycerol containing C18 fatty acids. These results suggested a higher contribution of these two pathways at the early stages of seed maturation. Lipidomic analysis of the content and acyl-group distribution of diacylglycerol and phosphatidyl-choline pools was compatible with the acyl content in triacylglycerol at the different maturation stages. Our data point to a model in which a strong temporal coordination between pathways and isoforms in each pathway, both at the expression and acyl-group incorporation, contribute to high erucic triacylglycerol accumulation in Pennycress. 000135487 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A09-20R$$9info:eu-repo/grantAgreement/ES/DGA/E25-23R$$9info:eu-repo/grantAgreement/ES/DGA/LMP194-21$$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-1265630B-I00 000135487 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es 000135487 590__ $$a4.8$$b2024 000135487 592__ $$a1.163$$b2024 000135487 591__ $$aPLANT SCIENCES$$b33 / 273 = 0.121$$c2024$$dQ1$$eT1 000135487 593__ $$aPlant Science$$c2024$$dQ1 000135487 594__ $$a8.8$$b2024 000135487 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000135487 700__ $$aLuján, María Ángeles 000135487 700__ $$aEscuín, José Manuel 000135487 700__ $$aSchilling, Marion 000135487 700__ $$aJouhet, Juliette 000135487 700__ $$0(orcid)0000-0001-7299-0187$$aSavirón, María$$uUniversidad de Zaragoza 000135487 700__ $$aLópez, M. Victoria 000135487 700__ $$aPicorel, Rafael 000135487 700__ $$0(orcid)0000-0002-8701-9745$$aJarne, Carmen$$uUniversidad de Zaragoza 000135487 700__ $$aCebolla, Vicente L. 000135487 700__ $$aAlfonso, Miguel 000135487 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica 000135487 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica 000135487 773__ $$g15 (2024), 1386023 [23 pp.]$$pFront. plant sci.$$tFrontiers in Plant Science$$x1664-462X 000135487 8564_ $$s10860720$$uhttps://zaguan.unizar.es/record/135487/files/texto_completo.pdf$$yVersión publicada 000135487 8564_ $$s2289583$$uhttps://zaguan.unizar.es/record/135487/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000135487 909CO $$ooai:zaguan.unizar.es:135487$$particulos$$pdriver 000135487 951__ $$a2026-01-12-13:00:23 000135487 980__ $$aARTICLE