Analysis of age-related changes in the left ventricular myocardium with multiphoton microscopy

Bueno, Juan M. ; Martínez-Ojeda, Rosa M. ; Pérez-Zabalza, María ; García-Mendívil, Laura ; Asensio, M. Carmen ; Ordovás, Laura ; Pueyo, Esther (Universidad de Zaragoza)

Resumen: Aging induces cardiac remodeling, resulting in an increase in the risk of suffering heart diseases, including heart failure. Collagen deposition increases with age and, together with sarcomeric changes in cardiomyocytes, may lead to ventricular stiffness. Multiphoton (MP) microscopy is a useful technique to visualize and detect variations in cardiac structures in a label free fashion. Here, we propose a method based on MP imaging (both two-photon excitation fluorescence (TPEF) and second harmonic generation (SHG) modalities) to explore and objectively quantify age-related structural differences in various components of cardiac tissues. Results in transmural porcine left ventricle (LV) sections reveal significant differences when comparing samples from young and old animals. Collagen and myosin SHG signals in old specimens are respectively 3.8x and >6-fold larger than in young ones. Differences in TPEF signals from cardiomyocyte were ∼3x. Moreover, the increased amount of collagen in old specimens results in a more organized pattern when compared to young LV tissues. Since changes in collagen and myosin are associated with cardiac dysfunction, the technique used herein might be a useful tool to accurately predict and measure changes associated with age-related myocardium fibrosis, tissue remodeling and sarcomeric alterations, with potential implications in preventing heart disease.

Idioma: Inglés

DOI: 10.1364/BOE.509227

Año: 2024

Publicado en: Biomedical Optics Express 15, 5 (2024), 3251-3264
ISSN: 2156-7085

Financiación: info:eu-repo/grantAgreement/ES/AEI/PID2020-113919RB-I00
Financiación: info:eu-repo/grantAgreement/ES/MCINN/PID2022-139859OB-I00
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2022-1405560B-I00
Financiación: info:eu-repo/grantAgreement/EUR/MICINN/TED2021-130459B-I00
Financiación: info:eu-repo/grantAgreement/ES/MINECO/PID2019-105674RB-I00

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Teoría Señal y Comunicac. (Dpto. Ingeniería Electrón.Com.)