000135738 001__ 135738
000135738 005__ 20240614091947.0
000135738 0247_ $$2doi$$a10.1071/RD23235
000135738 0248_ $$2sideral$$a138777
000135738 037__ $$aART-2024-138777
000135738 041__ $$aeng
000135738 100__ $$0(orcid)0000-0002-4670-3735$$aPeña-Delgado, Victoria$$uUniversidad de Zaragoza
000135738 245__ $$aGenotyping of rams based on melatonin receptor 1A gene polymorphisms: a tool in sire selection?
000135738 260__ $$c2024
000135738 5060_ $$aAccess copy available to the general public$$fUnrestricted
000135738 5203_ $$aSeveral polymorphisms in the melatonin receptor 1A gene (MTNR1A) have been related to reproductive performance in ovine.To investigate the effect of the RsaI and MnlI polymorphisms on ram seminal quality. Eighteen Rasa Aragonesa rams were genotyped for the RsaI (C/C, C/T, T/T) and MnlI (G/G, G/A, A/A) allelic variants of the MTNR1A gene. Individual ejaculates were analysed once a month throughout the whole year. Sperm motility, morphology, membrane integrity, levels of reactive oxygen species (ROS), phosphatidylserine (PS) inversion, DNA fragmentation and capacitation status were assessed. The effect of the season and polymorphisms on seminal quality was evaluated by mixed ANOVA. Both polymorphisms had an effect on membrane integrity and viable spermatozoa with low levels of ROS and without PS translocation, and RsaI also on motile and DNA-intact spermatozoa. An interaction between both polymorphisms was found, pointing to a negative effect on seminal quality of carrying the T or A allele in homozygosity. Differences were higher in the reproductive than in the non-reproductive season. Mutations substituting C by T and G by A at RsaI and MnlI polymorphic sites, respectively, in the MTNR1A gene in rams could decrease the seminal quality. Genotyping of rams based on melatonin receptor 1A could be a powerful tool in sire selection.
000135738 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000135738 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000135738 700__ $$0(orcid)0000-0002-9552-4709$$aNoya, Agustí$$uUniversidad de Zaragoza
000135738 700__ $$0(orcid)0000-0003-3929-5064$$aCarvajal-Serna, Melissa$$uUniversidad de Zaragoza
000135738 700__ $$aCanto, Francisco
000135738 700__ $$aSánchez, María Carmen
000135738 700__ $$aLetosa, Eva
000135738 700__ $$aVicente, Antonio
000135738 700__ $$aMorato, Ignacio
000135738 700__ $$aMacías, Ángel
000135738 700__ $$0(orcid)0000-0003-2827-3054$$aAbecia, José Alfonso$$uUniversidad de Zaragoza
000135738 700__ $$aCasao, Adriana
000135738 700__ $$0(orcid)0000-0002-2312-6402$$aPérez-Pe, Rosaura$$uUniversidad de Zaragoza
000135738 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología
000135738 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000135738 773__ $$g36, 9 (2024)$$pReprod. fertil. dev.$$tREPRODUCTION FERTILITY AND DEVELOPMENT$$x1031-3613
000135738 8564_ $$s1869523$$uhttps://zaguan.unizar.es/record/135738/files/texto_completo.pdf$$yVersión publicada
000135738 8564_ $$s2659737$$uhttps://zaguan.unizar.es/record/135738/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000135738 909CO $$ooai:zaguan.unizar.es:135738$$particulos$$pdriver
000135738 951__ $$a2024-06-14-08:59:06
000135738 980__ $$aARTICLE