135738 20260217205522.0 doi 10.1071/RD23235 sideral 138777 ART-2024-138777 eng Peña-Delgado, Victoria Universidad de Zaragoza (orcid)0000-0002-4670-3735 Genotyping of rams based on melatonin receptor 1A gene polymorphisms: a tool in sire selection? 2024 Access copy available to the general public Unrestricted Several polymorphisms in the melatonin receptor 1A gene (MTNR1A) have been related to reproductive performance in ovine.To investigate the effect of the RsaI and MnlI polymorphisms on ram seminal quality. Eighteen Rasa Aragonesa rams were genotyped for the RsaI (C/C, C/T, T/T) and MnlI (G/G, G/A, A/A) allelic variants of the MTNR1A gene. Individual ejaculates were analysed once a month throughout the whole year. Sperm motility, morphology, membrane integrity, levels of reactive oxygen species (ROS), phosphatidylserine (PS) inversion, DNA fragmentation and capacitation status were assessed. The effect of the season and polymorphisms on seminal quality was evaluated by mixed ANOVA. Both polymorphisms had an effect on membrane integrity and viable spermatozoa with low levels of ROS and without PS translocation, and RsaI also on motile and DNA-intact spermatozoa. An interaction between both polymorphisms was found, pointing to a negative effect on seminal quality of carrying the T or A allele in homozygosity. Differences were higher in the reproductive than in the non-reproductive season. Mutations substituting C by T and G by A at RsaI and MnlI polymorphic sites, respectively, in the MTNR1A gene in rams could decrease the seminal quality. Genotyping of rams based on melatonin receptor 1A could be a powerful tool in sire selection. info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 2.1 2024 ZOOLOGY 24 / 182 = 0.132 2024 Q1 T1 DEVELOPMENTAL BIOLOGY 20 / 39 = 0.513 2024 Q3 T2 REPRODUCTIVE BIOLOGY 27 / 43 = 0.628 2024 Q3 T2 0.327 2024 Animal Science and Zoology 2024 Q3 Biotechnology 2024 Q3 Reproductive Medicine 2024 Q3 Molecular Biology 2024 Q4 Genetics 2024 Q4 Developmental Biology 2024 Q4 Endocrinology 2024 Q4 2.1 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Noya, Agustí Universidad de Zaragoza (orcid)0000-0002-9552-4709 Carvajal-Serna, Melissa Universidad de Zaragoza (orcid)0000-0003-3929-5064 Canto, Francisco Sánchez, María Carmen Letosa, Eva Vicente, Antonio Morato, Ignacio Macías, Ángel Abecia, José Alfonso Universidad de Zaragoza (orcid)0000-0003-2827-3054 Casao, Adriana Pérez-Pe, Rosaura Universidad de Zaragoza (orcid)0000-0002-2312-6402 1002 819 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Zoología 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 36, 9 (2024) Reprod. fertil. dev. REPRODUCTION FERTILITY AND DEVELOPMENT 1031-3613 1869523 http://zaguan.unizar.es/record/135738/files/texto_completo.pdf Versión publicada 2659737 http://zaguan.unizar.es/record/135738/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:135738 articulos driver 2026-02-17-20:29:00 ARTICLE