000135757 001__ 135757 000135757 005__ 20240705134227.0 000135757 0247_ $$2doi$$a10.1016/j.foodcont.2024.110614 000135757 0248_ $$2sideral$$a138782 000135757 037__ $$aART-2024-138782 000135757 041__ $$aeng 000135757 100__ $$0(orcid)0000-0003-4460-3543$$aMerino, Natalia$$uUniversidad de Zaragoza 000135757 245__ $$aComparative analysis of commercial cleaning and disinfection formulations and protocols for effective eradication of biofilms formed by a Pseudomonas fluorescens strain isolated from a poultry meat plant 000135757 260__ $$c2024 000135757 5060_ $$aAccess copy available to the general public$$fUnrestricted 000135757 5203_ $$aPseudomonas spp. is the main genus of spoilage bacteria for meat stored under aerobic chilling conditions. Given their ability to adapt to various environmental conditions and to form biofilms, they are common among residential microbiota in the food industry. Biofilms are complex, structured microbial communities encased in a self-produced exopolysaccharide matrix. Resident biofilm bacteria show increased resistance against disinfectants and dynamic or hostile environments, and are therefore a persistent source of food contamination. Hence, there is a need to design and implement control strategies that are effective against biofilms in the food industry. In this study, we tested a series of commercial cleaning and disinfection agents applied by immersion or aerosolization against robust biofilms of a Pseudomonas fluorescens strain isolated from a poultry meat plant by comparing the sessile cell counts before and after the procedure. The single application of a) an alkaline or an enzymatic detergent, b) a disinfectant based on peracetic acid and hydrogen peroxide, or c) a detergent-disinfectant based on tertiary alkylamines or sodium hypochlorite, failed to completely eradicate the biofilm. However, two-step cleaning and disinfection procedures involving the application of a detergent (either alkaline or enzymatic at 50 °C) followed by the application of the disinfectant based on peracetic acid and hydrogen peroxide at the manufacturer's recommended concentrations did result in the complete eradication (p ≤ 0.05) of the robust biofilm. In addition, cleaning and disinfection procedures applied by aerosolization showed a notable efficacy (p ≤ 0.05) against surface-dried cells and sessile cells of P. fluorescens. Therefore, this approach could be regarded as a potentially viable alternative to commonly applied immersion techniques. 000135757 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-123404NB-I00$$9info:eu-repo/grantAgreement/ES/MCIU/FPU17-02441$$9info:eu-repo/grantAgreement/ES/DGA/A06-23R 000135757 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/ 000135757 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000135757 700__ $$aGarcía-Castillo, Carlota 000135757 700__ $$0(orcid)0000-0001-5053-8309$$aBerdejo, Daniel$$uUniversidad de Zaragoza 000135757 700__ $$0(orcid)0000-0003-3752-4660$$aPagán, Elisa 000135757 700__ $$0(orcid)0000-0002-7629-8101$$aGarcía-Gonzalo, Diego$$uUniversidad de Zaragoza 000135757 700__ $$0(orcid)0000-0002-0238-6328$$aPagán, Rafael$$uUniversidad de Zaragoza 000135757 7102_ $$12008$$2640$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Nutrición Bromatología 000135757 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos 000135757 773__ $$g164 (2024), 110614 [9 pp.]$$pFood control$$tFood Control$$x0956-7135 000135757 8564_ $$s1806083$$uhttps://zaguan.unizar.es/record/135757/files/texto_completo.pdf$$yVersión publicada 000135757 8564_ $$s2506990$$uhttps://zaguan.unizar.es/record/135757/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000135757 909CO $$ooai:zaguan.unizar.es:135757$$particulos$$pdriver 000135757 951__ $$a2024-07-05-12:56:00 000135757 980__ $$aARTICLE