000135763 001__ 135763
000135763 005__ 20240614091948.0
000135763 0247_ $$2doi$$a10.1016/j.ifset.2024.103707
000135763 0248_ $$2sideral$$a138794
000135763 037__ $$aART-2024-138794
000135763 041__ $$aeng
000135763 100__ $$aNadal, Laura$$uUniversidad de Zaragoza
000135763 245__ $$aExploring the mechanisms of Staphylococcus aureus pulsed electric fields resistance acquisition after exposure to heat and alkaline shocks
000135763 260__ $$c2024
000135763 5060_ $$aAccess copy available to the general public$$fUnrestricted
000135763 5203_ $$aThis study aimed to acquire a deeper knowledge of the mechanisms of PEF resistance development after the exposure of Staphylococcus aureus to sublethal alkaline and heat shocks, with a particular focus on the modifications of cell envelope properties and their impact on electroporation and its reversion. Both shocks significantly (p < 0.05) increased the surface negative charge but they barely affected surface hydrophobicity or membrane fluidity. This resulted in an increased electroporation threshold (≈ 2 kV/cm) for alkaline-shocked but not for heat-shocked cells. Heat and alkaline shock-dependent development of PEF resistance did not require de novo RNA, protein, or lipid synthesis. Addition of nisin (100 UI/mL) to the treatment medium not only counteracted the protective effect of sublethal shocks against PEF, but even increased the lethality of PEF treatments (up to 8.9-fold increase in Log cycles of inactivation) against heat-shocked and alkaline-shocked cells.
000135763 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A03-23R$$9info:eu-repo/grantAgreement/ES/MEFPD/FPU18-04898$$9info:eu-repo/grantAgreement/ES/UZ/JIUZ-2017-CIE-01
000135763 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000135763 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000135763 700__ $$0(orcid)0000-0002-5049-3646$$aCebrián, Guillermo$$uUniversidad de Zaragoza
000135763 700__ $$0(orcid)0000-0002-7971-4828$$aMañas, Pilar$$uUniversidad de Zaragoza
000135763 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000135763 773__ $$g95 (2024), 103707 [9 pp.]$$pInnov. food sci. emerg. technol.$$tINNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES$$x1466-8564
000135763 8564_ $$s1244976$$uhttps://zaguan.unizar.es/record/135763/files/texto_completo.pdf$$yVersión publicada
000135763 8564_ $$s2824879$$uhttps://zaguan.unizar.es/record/135763/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000135763 909CO $$ooai:zaguan.unizar.es:135763$$particulos$$pdriver
000135763 951__ $$a2024-06-14-08:59:52
000135763 980__ $$aARTICLE