000135771 001__ 135771
000135771 005__ 20240614091948.0
000135771 0247_ $$2doi$$a10.1186/s13018-024-04785-x
000135771 0248_ $$2sideral$$a138773
000135771 037__ $$aART-2024-138773
000135771 041__ $$aeng
000135771 100__ $$aEnrique, Cordero García-Galán
000135771 245__ $$aBiofilm on total joint replacement materials can be reduced through electromagnetic induction heating using a portable device
000135771 260__ $$c2024
000135771 5060_ $$aAccess copy available to the general public$$fUnrestricted
000135771 5203_ $$aBackground
periprosthetic joint infection is a serious complication following joint replacement. The development of bacterial biofilms bestows antibiotic resistance and restricts treatment via implant retention surgery. Electromagnetic induction heating is a novel technique for antibacterial treatment of metallic surfaces that has demonstrated in-vitro efficacy. Previous studies have always employed stationary, non-portable devices. This study aims to assess the in-vitro efficacy of induction-heating disinfection of metallic surfaces using a new Portable Disinfection System based on Induction Heating.
Methods
Mature biofilms of three bacterial species: S. epidermidis ATCC 35,984, S. aureus ATCC 25,923, E. coli ATCC 25,922, were grown on 18 × 2 mm cylindrical coupons of Titanium-Aluminium-Vanadium (Ti6Al4V) or Cobalt-chromium-molybdenum (CoCrMo) alloys. Study intervention was induction-heating of the coupon surface up to 70ºC for 210s, performed using the Portable Disinfection System (PDSIH). Temperature was monitored using thermographic imaging. For each bacterial strain and each metallic alloy, experiments and controls were conducted in triplicate. Bacterial load was quantified through scraping and drop plate techniques. Data were evaluated using non-parametric Mann-Whitney U test for 2 group comparison. Statistical significance was fixed at p ≤ 0.05.
Results
All bacterial strains showed a statistically significant reduction of CFU per surface area in both materials. Bacterial load reduction amounted to 0.507 and 0.602 Log10 CFU/mL for S. aureus on Ti6Al4V and CoCrMo respectively, 5.937 and 3.500 Log10 CFU/mL for E. coli, and 1.222 and 0.372 Log10 CFU/mL for S. epidermidis.
Conclusions
Electromagnetic induction heating using PDSIH is efficacious to reduce mature biofilms of S aureus, E coli and S epidermidis growing on metallic surfaces of Ti6Al4V and CoCrMo alloys.
000135771 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000135771 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000135771 700__ $$aMedel-Plaza, Marina
000135771 700__ $$aCorrea, John Jairo Aguilera
000135771 700__ $$0(orcid)0000-0001-8399-4650$$aSarnago, Héctor$$uUniversidad de Zaragoza
000135771 700__ $$0(orcid)0000-0001-7207-5536$$aAcero, Jesús$$uUniversidad de Zaragoza
000135771 700__ $$0(orcid)0000-0002-9655-5531$$aBurdio, José M.$$uUniversidad de Zaragoza
000135771 700__ $$0(orcid)0000-0002-1284-9007$$aLucía, Óscar$$uUniversidad de Zaragoza
000135771 700__ $$aEsteban, Jaime
000135771 700__ $$aGómez-Barrena, Enrique
000135771 7102_ $$15008$$2785$$aUniversidad de Zaragoza$$bDpto. Ingeniería Electrón.Com.$$cÁrea Tecnología Electrónica
000135771 773__ $$g19, 1 (2024), 304 [7 pp.]$$pJournal of orthopaedic surgery and research$$tJournal of Orthopaedic Surgery and Research$$x1749-799X
000135771 8564_ $$s1382204$$uhttps://zaguan.unizar.es/record/135771/files/texto_completo.pdf$$yVersión publicada
000135771 8564_ $$s2280864$$uhttps://zaguan.unizar.es/record/135771/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000135771 909CO $$ooai:zaguan.unizar.es:135771$$particulos$$pdriver
000135771 951__ $$a2024-06-14-09:00:02
000135771 980__ $$aARTICLE