000135827 001__ 135827
000135827 005__ 20240619140701.0
000135827 0247_ $$2doi$$a10.3791/66771
000135827 0248_ $$2sideral$$a138796
000135827 037__ $$aART-2024-138796
000135827 041__ $$aeng
000135827 100__ $$0(orcid)0000-0002-6600-1618$$aMoreno-Loshuertos, Raquel$$uUniversidad de Zaragoza
000135827 245__ $$aIsolation of Mitochondria for Mitochondrial Supercomplex Analysis from Small Tissue and Cell Culture Samples
000135827 260__ $$c2024
000135827 5060_ $$aAccess copy available to the general public$$fUnrestricted
000135827 5203_ $$aOver the last decades, the evidence accumulated about the existence of respiratory supercomplexes (SCs) has changed our understanding of the mitochondrial electron transport chain organization, giving rise to the proposal of the "plasticity model." This model postulates the coexistence of different proportions of SCs and complexes depending on the tissue or the cellular metabolic status. The dynamic nature of the assembly in SCs would allow cells to optimize the use of available fuels and the efficiency of electron transfer, minimizing reactive oxygen species generation and favoring the ability of cells to adapt to environmental changes.
More recently, abnormalities in SC assembly have been reported in different diseases such as neurodegenerative disorders (Alzheimer's and Parkinson's disease), Barth Syndrome, Leigh syndrome, or cancer. The role of SC assembly alterations in disease progression still needs to be confirmed. Nevertheless, the availability of enough amounts of samples to determine the SC assembly status is often a challenge. This happens with biopsy or tissue samples that are small or have to be divided for multiple analyses, with cell cultures that have slow growth or come from microfluidic devices, with some primary cultures or rare cells, or when the effect of particular costly treatments has to be analyzed (with nanoparticles, very expensive compounds, etc.). In these cases, an efficient and easy-to-apply method is required. This paper presents a method adapted to obtain enriched mitochondrial fractions from small amounts of cells or tissues to analyze the structure and function of mitochondrial SCs by native electrophoresis followed by in-gel activity assays or western blot.
000135827 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FEDER/E35-17R$$9info:eu-repo/grantAgreement/ES/DGA/LMP220_21$$9info:eu-repo/grantAgreement/ES/MICINN/PGC2018-095795-B-I00
000135827 540__ $$9info:eu-repo/semantics/embargoedAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000135827 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000135827 700__ $$0(orcid)0000-0001-8971-7355$$aFernández-Silva, Patricio$$uUniversidad de Zaragoza
000135827 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000135827 773__ $$g207 (2024)$$pJ. vis. exp.$$tJournal of visualized experiments : JoVE$$x1940-087X
000135827 8564_ $$s780065$$uhttps://zaguan.unizar.es/record/135827/files/texto_completo.pdf$$yPostprint$$zinfo:eu-repo/date/embargoEnd/2024-12-15
000135827 8564_ $$s2122509$$uhttps://zaguan.unizar.es/record/135827/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint$$zinfo:eu-repo/date/embargoEnd/2024-12-15
000135827 909CO $$ooai:zaguan.unizar.es:135827$$particulos$$pdriver
000135827 951__ $$a2024-06-19-13:22:37
000135827 980__ $$aARTICLE