135827 20260219073506.0 MULTIDISCIPLINARY SCIENCES 86 / 136 = 0.632 2024 Q3 T2 1.0 2024 Chemical Engineering (miscellaneous) 2024 Q2 Neuroscience (miscellaneous) 2024 Q3 Immunology and Microbiology (miscellaneous) 2024 Q3 Biochemistry, Genetics and Molecular Biology (miscellaneous) 2024 Q3 0.431 2024 2.0 2024 ARTICLE (orcid)0000-0001-8971-7355 Fernández-Silva, Patricio Universidad de Zaragoza ART-2024-138796 780065 http://zaguan.unizar.es/record/135827/files/texto_completo.pdf Postprint info:eu-repo/date/embargoEnd/2024-12-15 2122509 http://zaguan.unizar.es/record/135827/files/texto_completo.jpg?subformat=icon icon Postprint info:eu-repo/date/embargoEnd/2024-12-15 2026-02-17-20:37:41 info:eu-repo/semantics/openAccess by-nc https://creativecommons.org/licenses/by-nc/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Over the last decades, the evidence accumulated about the existence of respiratory supercomplexes (SCs) has changed our understanding of the mitochondrial electron transport chain organization, giving rise to the proposal of the "plasticity model." This model postulates the coexistence of different proportions of SCs and complexes depending on the tissue or the cellular metabolic status. The dynamic nature of the assembly in SCs would allow cells to optimize the use of available fuels and the efficiency of electron transfer, minimizing reactive oxygen species generation and favoring the ability of cells to adapt to environmental changes. More recently, abnormalities in SC assembly have been reported in different diseases such as neurodegenerative disorders (Alzheimer's and Parkinson's disease), Barth Syndrome, Leigh syndrome, or cancer. The role of SC assembly alterations in disease progression still needs to be confirmed. Nevertheless, the availability of enough amounts of samples to determine the SC assembly status is often a challenge. This happens with biopsy or tissue samples that are small or have to be divided for multiple analyses, with cell cultures that have slow growth or come from microfluidic devices, with some primary cultures or rare cells, or when the effect of particular costly treatments has to be analyzed (with nanoparticles, very expensive compounds, etc.). In these cases, an efficient and easy-to-apply method is required. This paper presents a method adapted to obtain enriched mitochondrial fractions from small amounts of cells or tissues to analyze the structure and function of mitochondrial SCs by native electrophoresis followed by in-gel activity assays or western blot. Access copy available to the general public Unrestricted 207 (2024) J. vis. exp. Journal of visualized experiments : JoVE 1940-087X 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. Isolation of Mitochondria for Mitochondrial Supercomplex Analysis from Small Tissue and Cell Culture Samples doi 10.3791/66771 sideral 138796 2024 (orcid)0000-0002-6600-1618 Moreno-Loshuertos, Raquel Universidad de Zaragoza eng oai:zaguan.unizar.es:135827 articulos driver info:eu-repo/grantAgreement/ES/DGA-FEDER/E35-17R info:eu-repo/grantAgreement/ES/DGA/LMP220_21 info:eu-repo/grantAgreement/ES/MICINN/PGC2018-095795-B-I00