000135837 001__ 135837
000135837 005__ 20240619140701.0
000135837 0247_ $$2doi$$a10.1002/mabi.202400073
000135837 0248_ $$2sideral$$a138865
000135837 037__ $$aART-2024-138865
000135837 041__ $$aeng
000135837 100__ $$aSawadkar, Prasad
000135837 245__ $$a3D porous binary composites of collagen, elastin, and fibrin proteins orchestrate adipose tissue regeneration
000135837 260__ $$c2024
000135837 5060_ $$aAccess copy available to the general public$$fUnrestricted
000135837 5203_ $$aThe objective for this study is to advance the development of a specialized biomaterial that can effectively facilitate the regeneration of adipose tissue. In prior studies, the assessment of collagen (Col), elastin (Ela), and fibrin (Fib) unary scaffolds has been conducted. However, it is important to note that native adipose tissue is comprised of a diverse array of extracellular matrix (ECM) constituents. To mimic this behavior, binary compositions of collagen, elastin, and fibrin are fabricated in a 1:1 ratio, resulting in the formation of Col/Ela, Col/Fib, and Ela/Fib composites through a customized fabrication procedure. The physical properties of these scaffolds are comprehensively analyzed using a range of material characterization techniques. Additionally, the biological properties of the scaffolds are investigated by examining the survival, proliferation, and phenotype of adipose‐derived stem cells. Subsequently, the aforementioned binary scaffolds are implanted into a rodent model for 28 days. the explants are analysed through X‐ray microtomography, histology, and immunohistochemistry. The findings of the study demonstrate that the utilization of binary combinations of Col/Ela, Col/Fib, and Ela/Fib has a discernible impact on the physical and biological characteristics of the scaffolds. Nevertheless, Ela/Fib exhibits characteristics that make it a suitable candidate for adipogenesis due to its notable upregulation of caveolin‐1 expression in both acellular and cellular cohorts. The combination of two natural polymers in this cell–material interaction has significantly enhanced the comprehension of adipogenesis.
000135837 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/RYC2021-033490-I
000135837 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000135837 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000135837 700__ $$aMandakhbayar, Nandin
000135837 700__ $$aPatel, Kapil D.
000135837 700__ $$aOwji, Nazanin
000135837 700__ $$aRajasekar, Poojitha
000135837 700__ $$aSarama, Roudin
000135837 700__ $$aLee, Jung-Hwan
000135837 700__ $$aKim, Hae-Won
000135837 700__ $$aKnowles, Jonathan
000135837 700__ $$0(orcid)0000-0001-7062-9099$$aGarcía-Gareta, Elena$$uUniversidad de Zaragoza
000135837 7102_ $$15001$$2065$$aUniversidad de Zaragoza$$bDpto. Ciencia Tecnol.Mater.Fl.$$cÁrea Cienc.Mater. Ingen.Metal.
000135837 773__ $$g(2024), [12 pp.]$$pMacromol. biosci.$$tMacromolecular Bioscience$$x1616-5187
000135837 8564_ $$s5273313$$uhttps://zaguan.unizar.es/record/135837/files/texto_completo.pdf$$yVersión publicada
000135837 8564_ $$s2561666$$uhttps://zaguan.unizar.es/record/135837/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000135837 909CO $$ooai:zaguan.unizar.es:135837$$particulos$$pdriver
000135837 951__ $$a2024-06-19-13:22:48
000135837 980__ $$aARTICLE